Evidence map›Paper›PMID 41449385›Full record

ArticleBMC molecular and cell biology2025

Molecular architecture of mesoderm cells across early to middle stage of human embryo development at single-cell resolution.

Wei Zhang, Haiyan Yu, Zhibin Zhang, Dandan Li, Yane Yang, Wei Shi, Wenting Li, Yan Jiang, Wenlong Hu, Zhipeng Zeng and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC molecular and cell biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Wei Zhang *Department of Clinical Laboratory, Peking University Shenzhen Hospital, Shenzhen, China.
Haiyan Yu *Pingshan District Peoples' Hospital of Shenzhen, Shenzhen, China.
Zhibin Zhang *Pingshan District Peoples' Hospital of Shenzhen, Shenzhen, China.
Dandan LiExperimental Center, Shenzhen Pingle Orthopedic Hospital (Shenzhen Pingshan Traditional Chinese Medicine Hospital), Shenzhen, China.
Yane YangShenzen Far East Women & Children Hospital, Shenzhen, China.
Wei ShiDepartment of Obstetrics and Gynecology, The Second Clinical Medical College of Jinan University, Shenzhen People's Hospital, Shenzhen, China.
Wenting LiSingleron Biotechnologies, Nanjing, China.
Yan JiangSingleron Biotechnologies, Nanjing, China.
Wenlong HuClinical Medical Research Center, The Second Clinical Medical College of Jinan University, Shenzhen People's Hospital, Shenzhen, China.
Zhipeng ZengClinical Medical Research Center, The Second Clinical Medical College of Jinan University, Shenzhen People's Hospital, Shenzhen, China.
Xinqiong LiuThe Second Clinical Medical College of Jinan University, Shenzhen People's Hospital, Shenzhen, China.
Zhanye ZhengSingleron Biotechnologies, Nanjing, China.
Minglin OuCentral Laboratory, The Second Affiliated Hospital of Guilin Medical University, Guilin, China. minglinou@glmc.edu.cn.
Donge TangClinical Medical Research Center, The Second Clinical Medical College of Jinan University, Shenzhen People's Hospital, Shenzhen, China. donge66@126.com.
Yong DaiClinical Medical Research Center, The Second Clinical Medical College of Jinan University, Shenzhen People's Hospital, Shenzhen, China. daiyong22@aliyun.com.

Funding

Guangdong Provincial Clinical Research Center for Laboratory Medicine 2023B110008Health System Research Project of Pingshan District in Shenzhen 202288Peking University Shenzhen Hospital Scientific Research Fund KYQD2025465Shenzhen High-level Hospital Construction Fund NAShenzhen High-level Hospital Construction Fund XKJS-2025-005the science and technology plan of Shenzhen JCYJ20220530152015035
6 · The paper itself

Abstract

backgroundThe differentiation of mesodermal cells (MCs) in the early stage of embryonic development contributes to the organogenesis of several core organs. However, the single-cell molecular architecture of MCs and the key molecular events during the differentiation remain unclear.

methodsWe performed single-cell RNA sequencing (RNA sequencing) and single-cell assay for transposase-accessible chromatin (ATAC-Seq) to analyze the developmental features of MCs to heart, kidney, spleen, liver, and brain in human embryos at gestational ages 7-17 weeks.

resultsWe found that EGR1 might be relevant to the differentiation of heterogeneous MC sub-clusters. Meanwhile, RPL10P9

conclusionsThese findings provide insights into the molecular architecture and lineage progression of MCs during early human embryonic organogenesis, offering a valuable reference for regenerative medicine and organ bioengineering.

Indexed as

Embryonic DevelopmentMesodermSingle-Cell AnalysisCell DifferentiationEmbryo, MammalianEndothelial CellsGene Expression Regulation, DevelopmentalHumansOrganogenesisEmbryo developmentMesoderm cellOrganogenesisSingle-cell assay for transposase-accessible chromatinSingle-cell RNA-seq

Identifiers

PMID41449385
PMCPMC12849250

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.