Evidence map›Paper›PMID 41449353›Full record

ArticleBMC genomic data2025

Whole genome sequence of Staphylococcus aureus strain 37 M isolated from cows with subclinical mastitis in Uganda.

Reagan Muhwezi, G R Neel, Emmanuel Eilu, Vidya Sankarapandian, Ambrose Shabohurira, Musoba Abubakar, Theophilus Pius, Priscilla Peter Dilli, Naheem Adekilekun Tijani, Danladi Makeri

Abstract read
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In one paragraph

Article in BMC genomic data, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Reagan MuhweziDepartment of Microbiology and Immunology, Kampala International University Western Campus, Ishaka, Uganda. muhwezi@kiu.ac.ug.
G R NeelDepartment of Microbiology and Immunology, School of Medicine, King Ceasor University, Kampala, Uganda.
Emmanuel EiluInstitute of Allied Health Sciences, Clarke International University, Kampala, Uganda.
Vidya SankarapandianDepartment of Microbiology and Immunology, School of Medicine, King Ceasor University, Kampala, Uganda.
Ambrose ShabohuriraDepartment of Microbiology and Immunology, Kampala International University Western Campus, Ishaka, Uganda.
Musoba AbubakarInstitute of Biomedical Research, Kampala International University Western Campus, Ishaka, Uganda.
Theophilus PiusDepartment of Medical Laboratory Science, Kampala International University Western Campus, Ishaka, Uganda.
Priscilla Peter DilliDepartment of Public Health, Kampala International University Western Campus, Ishaka, Uganda.
Naheem Adekilekun TijaniDepartment of Microbiology and Immunology, Kampala International University Western Campus, Ishaka, Uganda.
Danladi MakeriDepartment of Microbiology and Immunology, Kampala International University Western Campus, Ishaka, Uganda.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveThis study presents the whole genome sequence of Staphylococcus aureus strain 37M isolated from cows with subclinical mastitis in Kiruhura district, Uganda. The objective was to characterize the genome to understand the isolate's resistance and virulence potential. The genome sequence accompanying metadata are available under the accession GCA_052439145.1. DATA DESCRIPTION: The final assembled genome has a genome size of 2,795,296 bp, Contig N50 (53.2 kb), L50 16, Genome coverage 2.79534e + 06x, Assembly level contigs and a GC content of 32.5%. It contains 2,748 CDSs, 2,819 genes, 2,584 predicted protein-coding genes, 57 tRNA genes, 3 complete rRNAs (23 S), 5 non-coding RNAs and 159 Pseudo Genes. Quality analysis of the genome using CheckM analysis (v1.2.4) revealed 96.72% completeness, and 0.7% contamination. Average Nucleotide Identity (ANI) analysis revealed that the closest type strain to our isolate was Staphylococcus aureus (GCA_000330825.2) with an ANI of 99.04%, 92% assembly coverage, 93.3% type assembly coverage, confirming species-level identity and close relatedness. Screening with the Comprehensive Antibiotic Resistance Database (CARD) and the Virulence Factor Database (VFDB) revealed several genes conferring resistance to different classes of antibiotics: antibiotic (tet(K) and tet(38)- tetracyclines, mepA, aminoglycosides, fluoroquinolones, norA- Fluoroquinolones, LmrS- Macrolides, aminoglycosides, lincosamide, oxazolidinones and blaZ- Beta-lactams, regulatory gene (mepR, arlR and arlS). Virulence factors included: Adherence (icaA, icaB, icaC, icaD, icaR, clfA, ebp, fnbA, fnbB, map, sdrC, sbi, spa,), Secretion system (esaA, esaB, esaC, essA, essB, essC, esxA, esxB), Enzymes (cap8A, cap8B, cap8C, cap8D, cap8E, cap8F, cap8G, cap8P, cap8O, cap8N, cap8M, cap8L, mgrA, adsA, geh, srtB, hysA), Iron regulators (isdA, isdB, isdC, isdD, isdE, isdF isdG), Toxin (lukF-PV, lip, sspB, sspC) and Hemolysin (hlb, hld, aur, hlgA, hlgB, hlgC, hly/hla).

Indexed as

Genome, BacterialMastitis, BovineStaphylococcal InfectionsStaphylococcus aureusWhole Genome SequencingAnimalsAnti-Bacterial AgentsCattleFemalePhylogenyUgandaVirulenceVirulence FactorsAnti-Bacterial AgentsVirulence FactorsAntimicrobial-resistant genesBacterial genomicsGenomic annotationMinIon MK1B sequencingSubclinical mastitis

Identifiers

PMID41449353
PMCPMC12849609

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.