DNP enhanced solid-state NMR of lattice-like microcrystalline protein assemblies facilitated by co-assembly with dinitroxide-tagged proteins.

Christian M O'Neil, Lakshmi Bhai, Daniel W Conroy, Christopher P Jaroniec

Abstract read

In one paragraph

Article in Journal of magnetic resonance (San Diego, Calif. : 1997), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

4 authors.

Christian M O'NeilDepartment of Chemistry and Biochemistry, The Ohio State University, Columbus, OH, USA.

Lakshmi BhaiDepartment of Chemistry and Biochemistry, The Ohio State University, Columbus, OH, USA.

Daniel W ConroyDepartment of Chemistry and Biochemistry, The Ohio State University, Columbus, OH, USA.

Christopher P JaroniecDepartment of Chemistry and Biochemistry, The Ohio State University, Columbus, OH, USA. Electronic address: jaroniec.1@osu.edu.

Funding

Structural and dynamic studies of protein and protein-nucleic acid assemblies in health and diseaseR35GM156238 · NIGMS · OHIO STATE UNIVERSITY · PI Christopher P Jaroniec · 2025 to 2026

$1.1M

NIGMS NIH HHS R35 GM156238

6 · The paper itself

Abstract

Dynamic nuclear polarization (DNP) enables dramatic sensitivity enhancements of solid-state nuclear magnetic resonance (NMR) spectra for biological systems to be realized via polarization transfer from unpaired electrons of a polarizing agent, typically a nitroxide biradical, to nuclear spins of the system of interest. Here, we systematically investigate the prospect of recording DNP enhanced solid-state NMR spectra of hydrated lattice-like protein assemblies within sample formulations that are free of the commonly employed glycerol-based glassy solvent matrix containing exogenous polarizing agent molecules and instead consist of isotope-enriched protein of interest co-assembled with its dinitroxide-tagged structural analog incorporated as a dopant at low concentration. As a model system we use microcrystals of

Indexed as

Nitrogen OxidesNuclear Magnetic Resonance, BiomolecularProteinsCarbon IsotopesCrystallizationGlycerolMagnetic Resonance SpectroscopyModels, MolecularNitrogen IsotopesSpin LabelsCarbon IsotopesGlycerolNitrogen IsotopesNitrogen OxidesProteinsSpin LabelsDynamic nuclear polarizationMatrix-free DNP sample formulationProtein assemblyProtein structureSolid-state NMR spectroscopy

Identifiers

PMID41448118

DOI10.1016/j.jmr.2025.108011

PMCPMC12810707

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