Genomic characterization and comparative analysis of multidrug-resistant uropathogenic Escherichia coli strain U1 from Pakistan revealed ST131 as dominant clade.

Zaara Ishaq, Kainaat Khalil, Fiza Faiz, Uzair Haider, Samavi Nasir, Hajra Qayyum, Amjad Ali

Abstract readComparative Study

In one paragraph

Article in Antonie van Leeuwenhoek, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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7 authors.

Zaara IshaqIntegrative Biology Laboratory (IBG), Department of Microbiology and Biotechnology (MBT), Atta-ur-Rahman School of Applied Biosciences (ASAB), National University of Sciences and Technology (NUST), Sector H-12, Islamabad, 44000, Pakistan.

Kainaat KhalilIntegrative Biology Laboratory (IBG), Department of Microbiology and Biotechnology (MBT), Atta-ur-Rahman School of Applied Biosciences (ASAB), National University of Sciences and Technology (NUST), Sector H-12, Islamabad, 44000, Pakistan.

Fiza FaizIntegrative Biology Laboratory (IBG), Department of Microbiology and Biotechnology (MBT), Atta-ur-Rahman School of Applied Biosciences (ASAB), National University of Sciences and Technology (NUST), Sector H-12, Islamabad, 44000, Pakistan.

Uzair HaiderIntegrative Biology Laboratory (IBG), Department of Microbiology and Biotechnology (MBT), Atta-ur-Rahman School of Applied Biosciences (ASAB), National University of Sciences and Technology (NUST), Sector H-12, Islamabad, 44000, Pakistan.

Samavi NasirIntegrative Biology Laboratory (IBG), Department of Microbiology and Biotechnology (MBT), Atta-ur-Rahman School of Applied Biosciences (ASAB), National University of Sciences and Technology (NUST), Sector H-12, Islamabad, 44000, Pakistan.

Hajra QayyumIntegrative Biology Laboratory (IBG), Department of Microbiology and Biotechnology (MBT), Atta-ur-Rahman School of Applied Biosciences (ASAB), National University of Sciences and Technology (NUST), Sector H-12, Islamabad, 44000, Pakistan.

Amjad AliIntegrative Biology Laboratory (IBG), Department of Microbiology and Biotechnology (MBT), Atta-ur-Rahman School of Applied Biosciences (ASAB), National University of Sciences and Technology (NUST), Sector H-12, Islamabad, 44000, Pakistan. amjad.ali@asab.nust.edu.pk.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Uropathogenic Escherichia coli (UPEC) is a primary etiological agent of urinary tract infections (UTIs) worldwide. The emergence of multidrug-resistant (MDR) UPEC strains, especially the globally disseminated ST131 clone, poses a critical health threat in regions like Pakistan, where comprehensive genomic data is limited. This study performed an in-depth genomic characterization of a newly isolated MDR UPEC strain (U1) and conducted a comparative pangenome analysis of 73 UPEC genomes from Pakistan. The overall cohort exhibited an average genome size of 5.2 Mb, an average GC content of 50.6%, and an average of 5180 coding sequences. In silico genomic analysis identified U1 as a high-risk ST131 lineage member (O25:H4, phylogroup B2). The strain exhibited an MDR profile, supported by the prediction of key antibiotic resistance genes (ARGs), including bla

Indexed as

Drug Resistance, Multiple, BacterialEscherichia coli InfectionsGenome, BacterialUropathogenic Escherichia coliAnti-Bacterial AgentsGenomicsHumansPakistanPhylogenyUrinary Tract InfectionsVirulence FactorsAnti-Bacterial AgentsVirulence FactorsComparative genomicsMDRPakistanUPECUTIWGS

Identifiers

PMID41447408

DOI10.3390/antibiotics11111620

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