Evidence map›Paper›PMID 41445044›Full record

ArticleACS synthetic biology2026

A Simple and Versatile Cell-Free Expression Method for Producing Secondary Metabolites.

Jaime Lorenzo N Dinglasan, Namil Lee, Nam Ngoc Pham, Meghana Faltane, Marie Lynde, Katherine B Louie, Sangeeta Nath, Jay D Keasling, Hiroshi Otani, Nigel J Mouncey

Abstract read
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In one paragraph

Article in ACS synthetic biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Jaime Lorenzo N DinglasanUS Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United States.
Namil LeeCalifornia Institute for Quantitative Biosciences (QB3), University of California, Berkeley, California 94720, United States.
Nam Ngoc PhamEnvironmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United States.ORCID 0000-0001-7640-1001
Meghana FaltaneUS Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United States.
Marie LyndeUS Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United States.
Katherine B LouieUS Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United States.
Sangeeta NathUS Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United States.
Jay D KeaslingCalifornia Institute for Quantitative Biosciences (QB3), University of California, Berkeley, California 94720, United States.
Hiroshi OtaniUS Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United States.
Nigel J MounceyUS Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United States.ORCID 0000-0001-5380-1256

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Secondary metabolites are a major source of natural products with industrially relevant bioactivities. Lysate-based cell-free expression (CFE) is an emerging platform for accelerating the discovery and engineering of these natural products. While

Indexed as

Secondary MetabolismStreptomycesBacterial ProteinsBiological ProductsCell-Free SystemEscherichia coliMetabolic EngineeringStreptomyces lividansBacterial ProteinsBiological Productscell-free expressionnatural product synthesissecondary metabolitesStreptomycestype I polyketide synthase

Identifiers

PMID41445044
PMCPMC12814521

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.