Rapid laboratory detection of ustalic acid in freshly collected Tricholoma Kakishimeji using probe electrospray ionization tandem mass spectrometry (PESI-MS/MS).

Tetsuro Ito, Shintaro Aritaki, Mimori Tsuji, Wataru Aoki, Masashi Fukaya, Kaori Ryu, Katsuhiro Shiratake, Akiyoshi Yamada

Abstract read

PubMed Publisher

In one paragraph

Article in Journal of natural medicines, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Tetsuro ItoFaculty of Pharmacy, Gifu University of Medical Science, 4-3-3 Nijigaoka, Kani, Gifu, 509-0293, Japan. tito@u-gifu-ms.ac.jp.ORCID http://orcid.org/0000-0002-1842-1204

Shintaro AritakiGraduate School of Bio-Agricultural Sciences, Nagoya University, Furo- cho, Chikusa-ku, Nagoya, 464-8603, Japan.

Mimori TsujiGraduate School of Science and Technology, Department of Agriculture, Shinshu University, 8304 Minamiminowa, Nagano, 399-4598, Japan.

Wataru AokiDepartment of Mountain Ecosystem, Institute for Mountain Science, Shinshu University, 8304, Minamiminowa, Nagano, 399-4598, Japan.

Masashi FukayaFaculty of Pharmacy, Gifu University of Medical Science, 4-3-3 Nijigaoka, Kani, Gifu, 509-0293, Japan.

Kaori RyuFaculty of Pharmacy, Gifu University of Medical Science, 4-3-3 Nijigaoka, Kani, Gifu, 509-0293, Japan.

Katsuhiro ShiratakeGraduate School of Bio-Agricultural Sciences, Nagoya University, Furo- cho, Chikusa-ku, Nagoya, 464-8603, Japan. shira@agr.nagoya-u.ac.jp.

Akiyoshi YamadaGraduate School of Science and Technology, Department of Agriculture, Shinshu University, 8304 Minamiminowa, Nagano, 399-4598, Japan. akiyosh@shinshu-u.ac.jp.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Mushroom poisoning caused by Tricholoma kakishimeji (Kakishimeji) remains a public health concern in Japan. Ingestion of this mushroom induces acute gastrointestinal symptoms such as nausea, vomiting, abdominal pain, and diarrhea, and neurological symptoms have also been reported. The toxic principle, ustalic acid (UA), has previously been detected by liquid chromatography–mass spectrometry (LC–MS) and visualized by MALDI-imaging mass spectrometry, but these methods require laborious preparation and prolonged analysis. Here, we report a rapid and preparation-free method for detecting UA in freshly collected specimens using probe electrospray ionization tandem mass spectrometry (PESI-MS/MS). Fruiting bodies collected in October 2024 from the Iizuna Highlands (Nagano Prefecture) were analyzed in the laboratory, and UA was consistently detected within ~ 30 s in negative-ion mode with high sensitivity. This workflow highlights the potential for near-immediate chemical screening of toxic mushrooms, providing same-day diagnostic capability. Our findings demonstrate that PESI-MS/MS provides practical means for rapid chemical screening of freshly harvested mushrooms, offering timely support for toxicological investigations and food safety monitoring.

Indexed as

Spectrometry, Mass, Electrospray IonizationTandem Mass SpectrometryTricholomaFruiting Bodies, FungalMushroom PoisoningFood safetyProbe electrospray ionization tandem mass spectrometry (PESI-MS/MS)Rapid detectionToxic mushroomTricholoma kakishimejiUstalic acid

Identifiers

PMID41444467

DOI10.1007/s11418-025-01989-1

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.