Evidence map›Paper›PMID 41441944›Full record

ArticleWorld journal of microbiology & biotechnology2025

Combinatorial engineering strategies to reconstruct Aspergillus niger for improving its cellulase production efficiency.

Dongsheng Xue, Jueyan Chen, Dejun Duan, Zhiyong Chen, Xihuo You

Abstract read
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In one paragraph

Article in World journal of microbiology & biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Dongsheng XueKey Laboratory of Fermentation Engineering (Ministry of Education), National "111" Center for Cellular Regulation and Molecular Pharmaceutics, Cooperative Innovation Center of Industrial Fermentation (Ministry of Education & Hubei Province), Hubei University of Technology, Wuhan, 430068, PR China.
Jueyan ChenKey Laboratory of Fermentation Engineering (Ministry of Education), National "111" Center for Cellular Regulation and Molecular Pharmaceutics, Cooperative Innovation Center of Industrial Fermentation (Ministry of Education & Hubei Province), Hubei University of Technology, Wuhan, 430068, PR China.
Dejun DuanKey Laboratory of Fermentation Engineering (Ministry of Education), National "111" Center for Cellular Regulation and Molecular Pharmaceutics, Cooperative Innovation Center of Industrial Fermentation (Ministry of Education & Hubei Province), Hubei University of Technology, Wuhan, 430068, PR China.
Zhiyong ChenKey Laboratory of Fermentation Engineering (Ministry of Education), National "111" Center for Cellular Regulation and Molecular Pharmaceutics, Cooperative Innovation Center of Industrial Fermentation (Ministry of Education & Hubei Province), Hubei University of Technology, Wuhan, 430068, PR China.
Xihuo YouSchool of Bioengineering and Health, Wuhan Textile University, Wuhan, 430200, PR China. xhyouznhw@163.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Combinatorial engineering strategies were performed to improve cellulase production efficiencies of Aspergillus niger. Single functional cellulases in Aspergillus niger were knocked out with the exogenous multifunctional cellulases. Non-cellulase enzymes of phytase, β-mannanase and pectinase were deleted out from Aspergillus niger. The exogenous protein disulfide isomerase was expressed in Aspergillus niger. The cellulase activities from the reconstructed Aspergillus niger increased from 0.62 U/mL to 7.96 U/mL. Glucose released from corn stover increased from 12.5 g/L to 27.8 g/L. Deleting non-cellulase enzymes, expressing the multifunctional cellulase and exogenous protein disulfide isomerase could enhance cellulase production efficiencies of Aspergillus niger.

Indexed as

Aspergillus nigerCellulaseMetabolic EngineeringFungal ProteinsGlucoseZea maysCellulaseFungal ProteinsGlucoseAspergillus nigerMultifunctional cellulasesNon-cellulase enzymesProtein disulfide isomerase

Identifiers

PMID41441944

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.