Evidence map›Paper›PMID 41441687›Full record

ArticleVaccines2025

Humoral Response in Cattle Vaccinated with the Heterologous Sheeppox Virus Vaccine for Protection Against Lumpy Skin Disease: A Field Study.

Anna M Lyapina, Natalya V Kichemazova, Maxim S Lavrukhin, Yuri V Saltykov, Sergey S Zaytsev, Olga S Larionova, Valentina A Feodorova

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Vaccines, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Anna M LyapinaFundamental and Applied Research Lab, Saratov State University of Genetics, Biotechnology and Engineering Named After N.I. Vavilov, 335 Sokolovaya Str., 410005 Saratov, Russia.
Natalya V KichemazovaFundamental and Applied Research Lab, Saratov State University of Genetics, Biotechnology and Engineering Named After N.I. Vavilov, 335 Sokolovaya Str., 410005 Saratov, Russia.
Maxim S LavrukhinFundamental and Applied Research Lab, Saratov State University of Genetics, Biotechnology and Engineering Named After N.I. Vavilov, 335 Sokolovaya Str., 410005 Saratov, Russia.
Yuri V SaltykovFundamental and Applied Research Lab, Saratov State University of Genetics, Biotechnology and Engineering Named After N.I. Vavilov, 335 Sokolovaya Str., 410005 Saratov, Russia.
Sergey S ZaytsevFundamental and Applied Research Lab, Saratov State University of Genetics, Biotechnology and Engineering Named After N.I. Vavilov, 335 Sokolovaya Str., 410005 Saratov, Russia.
Olga S LarionovaDepartment for Microbiology and Biotechnology, Institute for Biotechnology, Saratov State University of Genetics, Biotechnology and Engineering Named After N.I. Vavilov, 335 Sokolovaya Str., 410005 Saratov, Russia.
Valentina A FeodorovaFundamental and Applied Research Lab, Saratov State University of Genetics, Biotechnology and Engineering Named After N.I. Vavilov, 335 Sokolovaya Str., 410005 Saratov, Russia.ORCID 0000-0002-3827-407X

Funding

Russian Science Foundation 22-16-00165-P
6 · The paper itself

Abstract

BACKGROUND/

objectivesLumpy skin disease (LSD) is a notifiable disease due to a marked potency for rapid spread and a significant negative economic impact on agriculture worldwide. As such, vaccination is considered the most effective way to control the disease in endemic countries, and the serological response to homologous LSDV-based vaccines is widely investigated. However, less is known about the seroconversion and duration of the immune response that is elicited by live attenuated heterologous vaccines based on the Sheeppox virus (SPPV) used for LSD prevention. This study aimed to investigate the humoral immune response in cattle immunized with a heterologous SPPV-based vaccine in the field.

methodsCommercial ELISA, based on P32 protein, as well as immunoblotting, were used to assess the antibody response in 6-month-old, 17-month-old, and older animals before and after the first immunization and revaccination.

resultsOnly a secondary immune response was detected when using commercial ELISA in revaccinated animals in each of the groups (83.3% and 30%, respectively). A comparative bioinformatic analysis proved a marked polymorphism of P32, derived from LSDV/SPPV/GTPV, which potentially resulted in negative responses in the ELISA. However, immunoblotting revealed a 100% seroconversion in vaccinated animals after the first vaccination and revaccination. Notably, specific antibodies were found in the sera of 80% of 6-month-old calves before the first vaccination, which had probably been passively transferred from their mothers, who were multiply vaccinated with SPPV-based vaccines.

conclusionsSeveral immunodominant antigens were able to induce a humoral immune response in cattle to the SPPV-based vaccine after passive and active immunization, serving as promising markers for a humoral immune response to heterologous vaccines.

Indexed as

capripoxviruscattleELISAheterologous vaccinehumoral immune responseimmunoblottingimmunoreactive proteinsLSDVP32SPPV

Identifiers

PMID41441687
PMCPMC12737495

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.