Construction of Spleen-Accumulated Polysorbate 20-Containing Ionizable Lipid Nanoparticles for mRNA Delivery.

Hanyu Liu, Siqi Li, Kexin Chen, Shuyi Yao, Xuefeng Tang, Xiaojun Han

Abstract read

In one paragraph

Article in Nanomaterials (Basel, Switzerland), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

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5 · Who and what money

Authors and funding

6 authors.

Hanyu LiuState Key Laboratory of Urban-Rural Water Resource and Environment, MIIT Key Laboratory of Critical Materials Technology for New Energy Conversion and Storage, Heilongjiang Provincial Joint Laboratory of Molecular Science (International Cooperation), School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150001, China.

Siqi LiState Key Laboratory of Urban-Rural Water Resource and Environment, MIIT Key Laboratory of Critical Materials Technology for New Energy Conversion and Storage, Heilongjiang Provincial Joint Laboratory of Molecular Science (International Cooperation), School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150001, China.

Kexin ChenState Key Laboratory of Urban-Rural Water Resource and Environment, MIIT Key Laboratory of Critical Materials Technology for New Energy Conversion and Storage, Heilongjiang Provincial Joint Laboratory of Molecular Science (International Cooperation), School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150001, China.

Shuyi YaoState Key Laboratory of Urban-Rural Water Resource and Environment, MIIT Key Laboratory of Critical Materials Technology for New Energy Conversion and Storage, Heilongjiang Provincial Joint Laboratory of Molecular Science (International Cooperation), School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150001, China.

Xuefeng TangState Key Laboratory of Urban-Rural Water Resource and Environment, MIIT Key Laboratory of Critical Materials Technology for New Energy Conversion and Storage, Heilongjiang Provincial Joint Laboratory of Molecular Science (International Cooperation), School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150001, China.

Xiaojun HanState Key Laboratory of Urban-Rural Water Resource and Environment, MIIT Key Laboratory of Critical Materials Technology for New Energy Conversion and Storage, Heilongjiang Provincial Joint Laboratory of Molecular Science (International Cooperation), School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150001, China.ORCID 0000-0001-8571-6187

Funding

National Natural Science Foundation of China 22174031National Natural Science Foundation of China 22374033Natural Science Foundation of Heilongjiang Province ZD2022B001

6 · The paper itself

Abstract

Messenger RNA therapy represents a transformative therapeutic in vaccine development, tumor immunotherapy, and genetic disease intervention. Polyethylene glycol (PEG) lipid, a key component of ionizable lipid nanoparticles (iLNPs) for mRNA delivery, and the PEG antibodies induced by PEG are associated with hypersensitivity and accelerated blood clearance. To address the above PEG-associated challenges, we systematically investigated polysorbate 20 (PS20) as an alternative for constructing PEG-free iLNPs. The formulation of PS20-incorporated iLNPs (PS20-iLNPs) for carrying mRNA was systematically investigated by analyzing the particle size, pKa, endosomal escape efficiency, and cellular internalization efficiency of iLNPs with different molar ratio PS20 content (0.5-5.0%). iLNPs with a relative molar ratio of 2.5% were identified as the optimal mRNA delivery carrier. This carrier exhibited excellent resistance to serum protein adsorption capacity, with serum stability 1.3-fold higher than PEG-iLNPs. At high lipid concentrations (2.7 mg/mL), the cell viability of PS20-iLNPs was maintained at 91.1%, which was 1.07-fold higher than PEG-iLNPs. Under serum interference, PS20-iLNPs achieved a transfection efficiency of 46.5%, marking a 10.6% improvement over PEG-iLNPs under identical conditions. Notably, PS20-iLNPs exhibited 24 times higher spleen accumulation than PEG-iLNPs. These findings highlight PS20 as a viable PEG substitute for developing PEG-free spleen-accumulated mRNA delivery platforms with enhanced therapeutic potential.

Indexed as

drug designionizable lipid nanoparticlesmRNA deliverypolysorbatespleen-accumulated

Identifiers

PMID41441458

DOI10.3390/nano15241844

PMCPMC12735810

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.