Evidence map›Paper›PMID 41440282›Full record

ArticleBiosensors2025

The Role of Surfactants in Stabilizing Fluorescence Anisotropy for Protein-Aptamer Binding Affinity Measurements.

Bhagya R Samarakoon, Susan L Bilderback, Rebecca J Whelan

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biosensors, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Bhagya R SamarakoonDepartment of Chemistry, University of Kansas, Lawrence, KS 66045, USA.ORCID 0009-0007-9079-0307
Susan L BilderbackDepartment of Chemistry, University of Kansas, Lawrence, KS 66045, USA.ORCID 0009-0006-3384-8931
Rebecca J WhelanDepartment of Chemistry, University of Kansas, Lawrence, KS 66045, USA.ORCID 0000-0002-9293-1528

Funding

Understanding the mechanobiology of stem cells in a microengineered 3D cardiac tissue environment with cardiomyopathyP20GM103638 · NIGMS · UNIVERSITY OF KANSAS LAWRENCE · PI LUNTE, SUSAN M · 2012 to 2021
$22.1M
Using Integrated Omics to Identify Dysfunctional Genetic Mechanisms Influencing Schizophrenia and Sleep DisturbancesP20GM130423 · NIGMS · UNIVERSITY OF KANSAS MEDICAL CENTER · PI Diane E Mahoney · 2019 to 2026
$21.5M
Women's Alzheimer's Risk Reduction in MidlifeP20GM152280 · NIGMS · UNIVERSITY OF KANSAS LAWRENCE · PI HEATHER R DESAIRE · 2024 to 2026
$10.0M
Synthetic Chemical Biology CoreP30GM145499 · NIGMS · UNIVERSITY OF KANSAS LAWRENCE · PI Susan M Lunte · 2022 to 2026
$6.9M
Targeted proteomics of MUC16 to enable early detection of ovarian cancer recurrenceR21CA267532 · NCI · UNIVERSITY OF NOTRE DAME · PI WHELAN, REBECCA JEAN · 2022 to 2023
$412k
NCI NIH HHS R21 CA267532NIGMS NIH HHS P20 GM103638NIGMS NIH HHS P20 GM130423NIGMS NIH HHS P20 GM152280NIGMS NIH HHS P30 GM145499NIH HHS 1P20GM103638-25NIH HHS 1P20GM130423-25NIH HHS 1P20GM152280-25NIH HHS 5R21CA267532-25Tina's Wish n/a
6 · The paper itself

Abstract

Fluorescence Anisotropy (FA) is a sensitive and efficient technique for quantifying biomolecular interactions, offering advantages such as minimal sample requirements and elimination of separation of bound from unbound species. Thus, it is well suited for aptamer-protein binding affinity studies. However, accurately determining equilibrium dissociation constants (

Indexed as

Aptamers, NucleotideBiosensing TechniquesFluorescence PolarizationSurface-Active AgentsHumansProtein BindingThrombinAptamers, NucleotideSurface-Active AgentsThrombinthrombin aptameraptamer–protein binding affinityfluorescence anisotropy (FA)surfactantsTriton X-100Tween 20

Identifiers

PMID41440282
PMCPMC12730314

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.