Evaluation of chrysin incorporated chitosan-based scaffold for bone regeneration in rat models.

Background: Periodontal osseous defects, which are the sequelae of periodontitis, are often treated with open flap debridement and bone replacement grafts (BRG). Although various grafts are available for clinical use, none of them fulfill the ideal properties of the BRG, which underscores the need for newer regenerative materials. One such material, which is highly investigated, is the flavonoid. Flavonoids are phytochemical compounds that possess anti inflammatory, antioxidant, osteogenic, osteoprotective, and anti tumorogenic properties. Chrysin, a flavonoid obtained from the passion flower, honey, and mushrooms, has anti inflammatory properties, osteogenic potential, and accelerates wound healing. Thus, this study aims to analyze bone regeneration in a critical size defect treated with chrysin incorporated modified chitosan scaffold in a rat model. Materials and Methods: In this study, 18 male Wistar rats were enrolled, and they were allocated into three groups: Group I (defect only), Group II (defect treated with chitosan scaffolds), and Group III (defect treated with chrysin incorporated chitosan scaffolds). The outcomes were analyzed with radiographic and histologic parameters. Results: On comparison between the groups, there was a statistically significant increase in the loose connective tissue in the chrysin group (Group III) ( Conclusions: Thus, the present study reported that the chrysin molecule incorporated into the scaffold enhanced early wound healing and woven bone apposition. There was no statistically significant difference between the chrysin incorporated scaffold and the chitosan only scaffold, but the results showed a positive response in the chrysin incorporated group (Group III) when compared to the other 2 group.