Development of a Multilayered Prognostic Model for Wilms' Tumor Based on Characteristic Lymphocyte Genes.

Objective: To develop a prognostic nomogram for Wilms' tumor (WT) integrating genetic and clinical factors to improve evaluation accuracy and clinical utility. Methods: RNA sequencing (RNA-seq) data from 125 WT patients and single-cell RNA (scRNA-seq) data from 2437 samples were analyzed using bioinformatics tools for data processing, including normalization and scaling with SCTransform, and cell clustering with Seurat. Principal component analysis (PCA) and Uniform Manifold Approximation and Projection (UMAP) were utilized for data visualization. Differential gene expression analysis identified pivotal genes for the Genetic Feature Prognostic Model for WT (GPM-WT). Univariate Cox regression analysis refined this model by incorporating clinical prognostic indicators. Survival analysis, Cox regression, and ROC curve assessments evaluated these models' prognostic capabilities. Immune cell infiltration and drug sensitivity were quantified, linking these to patient risk categories. Results: Six prognostic lymphocyte genes ( Conclusions: The study developed an integrated LGCPN-WT model, significantly enhancing survival prediction accuracy and clinical utility for WT, thus supporting personalized treatment approaches.