Evidence map›Paper›PMID 41438071›Full record

ArticleiScience2025

Site-specific ERα phosphorylation determines sex-dependent metabolic, reproductive, and body-compositional phenotypes in mice.

Binghao Zou, Jarvis Williams, Madeleine B Landau, Weiqiang Lin, Sallie Fell, Ziqi Yang, MaryJane Jones, Robert Blair, Chad H Steele, Pratik Khare and 4 more

Erratum issuedAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in iScience, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

14 authors.

Binghao ZouDepartment of Structural and Cellular Biology, Tulane University School of Medicine, New Orleans, LA, USA.
Jarvis WilliamsDepartment of Structural and Cellular Biology, Tulane University School of Medicine, New Orleans, LA, USA.
Madeleine B LandauDepartment of Structural and Cellular Biology, Tulane University School of Medicine, New Orleans, LA, USA.
Weiqiang LinTulane Center for Biomedical Informatics and Genomics, Deming Department of Medicine, Tulane University School of Medicine, New Orleans, LA, USA.
Sallie FellDepartment of Structural and Cellular Biology, Tulane University School of Medicine, New Orleans, LA, USA.
Ziqi YangDepartment of Structural and Cellular Biology, Tulane University School of Medicine, New Orleans, LA, USA.
MaryJane JonesDepartment of Microbiology and Immunology, Tulane University School of Medicine, New Orleans, LA, USA.
Robert BlairDepartment of Pathology and Laboratory Medicine, Tulane University School of Medicine, New Orleans, LA, USA.
Chad H SteeleDepartment of Microbiology and Immunology, Tulane University School of Medicine, New Orleans, LA, USA.
Pratik KhareGigantest, Baltimore, MD, USA.
Cissy ZhangGigantest, Baltimore, MD, USA.
Anne LeGigantest, Baltimore, MD, USA.
Muralidharan AnbalaganDepartment of Structural and Cellular Biology, Tulane University School of Medicine, New Orleans, LA, USA.
Brian G RowanDepartment of Structural and Cellular Biology, Tulane University School of Medicine, New Orleans, LA, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Estrogen receptor alpha (ERα) phosphorylation regulates receptor activity and tissue-specific gene expression. We generated serine (S) to alanine (A) phosphorylation-deficient knock-in mice targeting two conserved ERα sites, S171 and S216, to examine their physiological roles. ERα S216A females were subfertile, with ∼30% smaller litters and diminished uterine growth in response to estradiol (E2). Single-cell spatial transcriptomics revealed a disrupted E2-regulated transcriptome in the myometrium. Metabolic profiling revealed the suppression of glycolytic and redox pathways in ERα S216A mice, with males exhibiting reduced adiposity and increased lean mass. Skeletal analysis revealed opposing effects: ERα S216A females exhibited reduced femoral bone density, while ERα S171A females showed an increase. These data demonstrate critical roles for site-specific ERα phosphorylation in modulating receptor levels and activity, as well as gene expression, which have a profound impact on murine body composition, fertility, and metabolism.

Indexed as

cell biologymetabolomicstranscriptomics

Identifiers

PMID41438071
PMCPMC12719750

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.