ArticleClinical and experimental reproductive medicine2026
Differences in CXCR4 expression between day 5 and day 6 euploid blastocysts: A predictor of implantation potential in single embryo transfer.
Article in Clinical and experimental reproductive medicine, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
objectiveThis study aimed to compare the expression of implantation-related genes in trophectoderm cells between normally developing (day 5) and delayed (day 6) euploid blastocysts.
methodsTrophectoderm biopsies were performed on blastocysts at either day 5 or day 6 of development. Preimplantation genetic testing for aneuploidy was conducted using next-generation sequencing. The expression levels of GAPDH, CXCR4, CXCL12, HSD3B1, HSD17B1, ITGAV, LAMA1, and MUC15 were measured from the remaining trophectoderm samples of euploid blastocysts using real-time quantitative polymerase chain reaction. Differences in gene expression levels between day 5 and day 6 euploid blastocysts were analyzed with the 2-ΔΔCt method. Pregnancy outcomes were assessed following single embryo transfer.
resultsEuploid blastocysts from day 5 (n=10) and day 6 (n=10) were included in the analysis. The expression level of CXCR4 was significantly lower in day 5 blastocysts compared to day 6 blastocysts (p=0.031). CXCL12, HSD17B1, ITGAV, and LAMA1 exhibited upregulated expression, while HSD3B1 and MUC15 showed downregulation in both day 5 and day 6 blastocysts, without significant differences between groups. Although gene expression did not differ significantly between pregnant and non-pregnant groups, early upregulation of CXCR4 was observed in the non-pregnant group following day 5 embryo transfer.
conclusionAs the only gene associated with implantation, CXCR4 displayed a distinctive expression profile that increased during blastocyst development. Aberrant CXCR4 expression at specific stages of blastocyst development may influence pregnancy outcomes.
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