ArticleBMC infectious diseases2025
Prevalence of quorum-sensing genes (lasI, lasR, RhlI and rhlR) in biofilm-producing Pseudomonas aeruginosa clinical isolates in Northern Iran.
Article in BMC infectious diseases, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- Integrative Systems-Level Transcriptomic Network Analysis Identifies Candidate Genes Associated with Biofilm Formation and Virulence inInternational journal of molecular sciences · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionThe quorum sensing (QS) systems are involved in biofilm formation ability of Pseudomonas aeruginosa, as key factors in the development of infections. Therefore, the aim of this study was to determine the frequency of significant QS encoding genes, including lasI, lasR, rhlI, and rhlR, in clinical isolates of P. aeruginosa. MATERIALS AND
methodsThis study was performed on 100 clinical isolates of P. aeruginosa that were identified by biochemical, microbiological, and PCR tests. The biofilm formation assay was done by microtiter plate method. Alkaline Lysis was used to extract the genomic DNAs. Then, the PCR method was used to identify the 16 S rRNA gene and the frequency of the lasR, lasI, rhlR, and rhlI genes.
resultsAmong 89 biofilm-producer isolates, 48 (53.93%), 17 (19.1%), and 24 (26.96%) showed a strong, moderate, and weak biofilm formation ability, respectively. The lasI gene was identified in 94% of the isolates and the rhlR gene was presented in 99% of the isolates, while 100% of the isolates carried the lasR and rhlI genes. Among the 6 isolates lacking the lasI gene, 3, 1, 1, and 1 isolates were collected from the ICU, emergency, burn, and surgical departments, respectively. In addition, 3, 1, 1, and 1 isolates lacking the lasI gene were collected from sputum, urine, wound, and catheter samples, respectively. One strain lacking the rhlR gene was also isolated from a wound sample in burn department. In this study, 94% of the isolates had all 4 genes tested, while 100% of the isolates carried at least 3 QS genes.
conclusionConsidering that all biofilm-producing isolates were collected from hospitalized patients with active infections, it can be concluded that the presence of QS genes in the development of acute infections caused by this organism has been proven in this study.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.