Evidence map›Paper›PMID 41436764›Full record

ArticleScientific reports2025

Phylodynamic analysis reveals disparate transmission dynamics of Mycobacterium tuberculosis complex lineages in Botswana.

Qiao Wang, Ivan Barilar, Volodymyr M Minin, Chawangwa Modongo, Patrick K Moonan, Alyssa Finlay, Rosanna Boyd, John E Oeltmann, Tuduetso L Molefi, Nicola M Zetola and 3 more

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Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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2 · The registry

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3 · Its place in the literature

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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

13 authors.

Qiao WangDepartment of Epidemiology, Fielding School of Public Health, University of California Los Angeles, Los Angeles, CA, USA.
Ivan BarilarMolecular Mycobacteriology, Forschungszentrum Borstel, Borstel, Germany.
Volodymyr M MininDepartment of Statistics, University of California Irvine, Irvine, CA, USA.
Chawangwa ModongoVictus Global Botswana Organisation, Gaborone, Botswana.
Patrick K MoonanDivision of Global HIV and Tuberculosis, Centers for Disease Control and Prevention, Atlanta, GA, USA.
Alyssa FinlayDivision of Tuberculosis Elimination, Centers for Disease Control and Prevention, Atlanta, GA, USA.
Rosanna BoydDivision of Tuberculosis Elimination, Centers for Disease Control and Prevention, Atlanta, GA, USA.
John E OeltmannDivision of Global HIV and Tuberculosis, Centers for Disease Control and Prevention, Atlanta, GA, USA.
Tuduetso L MolefiNational TB Program, Ministry of Health, Gaborone, Botswana.
Nicola M ZetolaAugusta University School of Medicine, Augusta, GA, USA.
Timothy F BrewerDepartment of Epidemiology, Fielding School of Public Health, University of California Los Angeles, Los Angeles, CA, USA.
Stefan NiemannMolecular Mycobacteriology, Forschungszentrum Borstel, Borstel, Germany.
Sanghyuk S ShinSue & Bill Gross School of Nursing, University of California Irvine, Irvine, CA, USA. ssshin2@hs.uci.edu.

Funding

Improved understanding of TB transmission by accounting for within-host heterogeneity of M. tuberculosis: A population-based molecular epidemiology study in a high HIV prevalent settingR01AI147336 · NIAID · UNIVERSITY OF CALIFORNIA-IRVINE · PI SHIN, SANGHYUK SAM · 2020 to 2024
$3.3M
Dynamics of TB and MDR TB transmission in areas with high HIV prevalenceR01AI097045 · NIAID · UNIVERSITY OF PENNSYLVANIA · PI ZETOLA, NICOLA MARIO · 2011 to 2015
$2.9M
National Institute of Allergy and Infectious Diseases R01AI097045National Institute of Allergy and Infectious Diseases R01AI147336NIAID NIH HHS R01 AI097045NIAID NIH HHS R01 AI147336
6 · The paper itself

Abstract

Tuberculosis epidemics have traditionally been conceptualized as arising from a single uniform pathogen. However, Mycobacterium tuberculosis complex (Mtbc), the pathogen causing tuberculosis in humans, encompasses multiple lineages exhibiting genetic and phenotypic diversity that may be responsible for heterogeneity in tuberculosis transmission. We analysed a population-based dataset of 1,354 Mtbc whole-genome sequences collected over four years in Botswana, a country with high HIV and tuberculosis burden. We identified Lineage 4 (L4) as the most prevalent (87.4%), followed by L1 (6.4%), L2 (5.3%), and L3 (0.9%). Within L4, multiple sublineages were identified, with L4.3.4 being the predominant sublineage. Phylodynamic analysis revealed that L4.3.4 expanded steadily from the late 1800s to early 2000s. Conversely, L1, L4.4, and L4.3.2 showed population trajectories closely aligned with the HIV epidemic. Meanwhile, L2 saw rapid expansion throughout most of the 20th century but declined sharply in the early 1990s. Additionally, pairwise genome comparison of Mtbc highlighted differences in clustering proportions due to recent transmission at the sublineage level. These findings emphasize the diverse transmission dynamics of strains of different Mtbc lineages and highlight the potential for phylodynamic analysis of routine sequences to refine our understanding of lineage-specific behaviors.

Indexed as

Mycobacterium tuberculosisTuberculosisBotswanaGenetic VariationGenome, BacterialHIV InfectionsHumansPhylogenyWhole Genome Sequencing

Identifiers

PMID41436764
PMCPMC12804184

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