Evidence map›Paper›PMID 41436534›Full record

ArticleScientific reports2025

Morphological characterization, mitochondrial genome assembly, and phylogenetic reconstruction of Branchiostoma malayanum within Branchiostomatidae.

Lei Xu, Yafang Li, Quehui Tang, Haigang Chen, Zhenzhao Tang, Pujiang Huang, Jiajia Ning, Lianggen Wang, Shuangshuang Liu, Delian Huang and 2 more

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Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

12 authors.

Lei Xu *South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510300, China.
Yafang Li *South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510300, China.
Quehui TangSouth China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510300, China.
Haigang ChenSouth China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510300, China.
Zhenzhao TangSouth China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510300, China.
Pujiang HuangShenzhen Ocean Development Promotion Center, Shenzhen, China.
Jiajia NingSouth China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510300, China.
Lianggen WangSouth China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510300, China.
Shuangshuang LiuSouth China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510300, China.
Delian HuangSouth China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510300, China.
Chunbin JiaShenzhen Ocean Development Promotion Center, Shenzhen, China. jcbsz@163.com.
Feiyan DuSouth China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510300, China. feiyanegg@163.com.

Funding

Central Public-interest Scientific Institution Basal Research Fund, CAFS N0.2023TD16Guangdong Basic and Applied Basic Research Foundation 2022B1515250005In-situ monitoring of benthic communities in typical nearshore habitats of Shenzhen NO. SYF[2023]46Investigation and Evaluation System Construction of Aquatic Biodiversity in Shenzhen coastal waters NO. SYF[2022]40the foundation of Guangdong Provincial Observation and Research Station for Coastal Upwelling Ecosystem CUE202305
6 · The paper itself

Abstract

Branchiostoma malayanum is a poorly studied amphioxus species within the family Branchiostomatidae, with limited genomic resources available to support its taxonomic and evolutionary characterization. In this study, we present the first complete mitochondrial genome of B. malayanum and assess its phylogenetic position through a combination of morphological characterization, mitogenomic analysis, and comparative genetic divergence. The assembled mitogenome is 15,154 base pairs in length, encoding 13 protein-coding genes, 22 tRNAs, and 2 rRNAs, with conserved gene order and a strong AT bias (63.46%). Codon usage analysis revealed compositional asymmetry and functional constraints typical of cephalochordates. Morphological measurements showed a mixture of conserved segmental traits and variable external features influenced by developmental allometry. Comparative analysis of four mitochondrial markers (COX1, Cytb, 12 S rRNA, 16 S rRNA) demonstrated substantial intergeneric divergence among Branchiostoma, Asymmetron, and Epigonichthys, and supported the distinctiveness of B. malayanum from its congeners. Phylogenetic reconstruction based on complete mitogenomes positioned B. malayanum within a derived clade of Branchiostoma, closely related to B. belcheri and B. japonicum. These findings confirm the independent evolutionary status of B. malayanum and highlight the value of mitogenomic data in resolving cephalochordate diversity and systematics.

Indexed as

Genome, MitochondrialLanceletsPhylogenyAnimalsCodon UsageEvolution, MolecularRNA, RibosomalRNA, TransferRNA, RibosomalRNA, TransferBranchiostoma malayanumBranchiostomatidaeMitochondrial genomeMorphological traitsPhylogenetic

Identifiers

PMID41436534
PMCPMC12728174

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