Evidence map›Paper›PMID 41436063›Full record

ArticleACS synthetic biology2026

Recoded Bacteriophage Genome for Bio-Orthogonal-Enabled Concentration and Detection of

David R Parker, Andrew P Sikkema, Ranee K Anderson, Gregory J S Lohman, Sam R Nugen

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS synthetic biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Scarless one-tube genome assemblyRSC chemical biology · 2026
    Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

David R ParkerDepartment of Food Science, Cornell University, Ithaca, New York 14853, United States.
Andrew P SikkemaNew England Biolabs, Ipswich, Massachusetts 01938, United States.
Ranee K AndersonDepartment of Food Science, Cornell University, Ithaca, New York 14853, United States.
Gregory J S LohmanNew England Biolabs, Ipswich, Massachusetts 01938, United States.ORCID 0000-0002-0638-7555
Sam R NugenDepartment of Food Science, Cornell University, Ithaca, New York 14853, United States.ORCID 0000-0003-3638-1776

Funding

Accelerating phage evolution and tools via synthetic biology and machine learningR01EB027895 · NIBIB · CORNELL UNIVERSITY · PI NUGEN, SAM R · 2019 to 2024
$2.6M
NIBIB NIH HHS R01 EB027895
6 · The paper itself

Abstract

Modern genome editing methods permit the flexible modification of organisms at the genome level. However, bacteriophages, despite their small genomes, pose unique challenges due to the need to edit during their infection cycle, then select/screen for the modified genomes against a background of the wild type phage. Direct genome synthesis enabled by High-Complexity Golden Gate Assembly (HC-GGA) offers an alternative approach that permits rapid, accurate, and flexible genome modification. Here, we demonstrate HC-GGA's bacteriophage engineering potential, particularly in addressing the public health challenge of detecting hazardous pathogens and nonpathogenic bacteria as indicators of fecal contamination (indicator organisms) in water supplies. A bacteriophage-based biosensor was developed by recoding the genome to enable

Indexed as

BacteriophagesDrinking WaterEscherichia coliGenome, ViralBiosensing TechniquesDrinking Waterbacteriophage engineeringbioorthogonal labelingenvironmental biosensorgolden gate assemblyNanoLuc reporternoncanonical amino acid incorporation

Identifiers

PMID41436063
PMCPMC12814771

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.