Evidence map›Paper›PMID 41435832›Full record

ArticleCell reports. Medicine2026

In mouse and in vitro models, bowel preparation promotes pathogen colonization, translocation, and exacerbation of inflammation.

Charlotte A Clayton, Imogen Porter, Brian D Deng, Giselle McCallum, Apsara Srinivas, Claire Sie, Jerry Y He, Alexander D Pei, Dominique Tertigas, Deanna M Pepin and 6 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports. Medicine, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Charlotte A ClaytonDepartment of Microbiology & Immunology, University of British Columbia, Vancouver, BC V6T1Z3, Canada.
Imogen PorterGenome Science & Technology, University of British Columbia, Vancouver, BC V6T1Z4, Canada.
Brian D DengDepartment of Microbiology & Immunology, University of British Columbia, Vancouver, BC V6T1Z3, Canada.
Giselle McCallumDepartment of Microbiology & Immunology, University of British Columbia, Vancouver, BC V6T1Z3, Canada.
Apsara SrinivasDepartment of Microbiology & Immunology, University of British Columbia, Vancouver, BC V6T1Z3, Canada.
Claire SieDepartment of Microbiology & Immunology, University of British Columbia, Vancouver, BC V6T1Z3, Canada.
Jerry Y HeDepartment of Microbiology & Immunology, University of British Columbia, Vancouver, BC V6T1Z3, Canada.
Alexander D PeiDepartment of Microbiology & Immunology, University of British Columbia, Vancouver, BC V6T1Z3, Canada.
Dominique TertigasBiochemistry and Biomedical Sciences, McMaster University, Hamilton, ON L8S4L8, Canada.
Deanna M PepinDepartment of Microbiology & Immunology, University of British Columbia, Vancouver, BC V6T1Z3, Canada.
Touran FardeenDepartment of Medicine, Division of Gastroenterology and Hepatology, Stanford University School of Medicine, Stanford, CA 94305, USA.
Katharine M NgDepartment of Microbiology & Immunology, University of British Columbia, Vancouver, BC V6T1Z3, Canada.
Sidhartha R SinhaDepartment of Medicine, Division of Gastroenterology and Hepatology, Stanford University School of Medicine, Stanford, CA 94305, USA.
Michael G SuretteBiochemistry and Biomedical Sciences, McMaster University, Hamilton, ON L8S4L8, Canada; Department of Medicine, McMaster University, Hamilton, ON L8N3Z5, Canada.
Bruce A VallanceDepartment of Pediatrics, Division of Gastroenterology, Hepatology and Nutrition, British Columbia Children's Hospital Research Institute, Vancouver, BC V6H3V4, Canada.
Carolina TropiniDepartment of Microbiology & Immunology, University of British Columbia, Vancouver, BC V6T1Z3, Canada; School of Biomedical Engineering, University of British Columbia, Vancouver, BC V6T2B9, Canada; Humans and the Microbiome Program, Canadian Institute for Advanced Research (CIFAR), Toronto, ON M5G1M1, Canada. Electronic address: carolina.tropini@ubc.ca.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In the United States, an estimated 14 million colonoscopies are performed yearly, each requiring patients to undergo bowel preparation, a laxative cleansing of the intestine's luminal contents. Despite its widespread use, the effects of bowel preparation on gut physiology and susceptibility to pathogens remain poorly understood, particularly in individuals with compromised gut health. Using mouse and in vitro models, we find that bowel preparation with the laxative polyethylene glycol rapidly disrupts the gut, transiently increasing susceptibility to infection by Salmonella Typhimurium, including a non-motile mutant, and by gut pathobionts derived from ulcerative colitis microbiota. Bowel preparation also facilitates bacterial translocation to extraintestinal sites (mesenteric lymph nodes, liver, and spleen) and exacerbates inflammation in a chemically induced colitis model. Although these findings are preclinical, they suggest that bowel preparation may have underappreciated risks in vulnerable populations and warrant further clinical investigation.

Indexed as

Bacterial TranslocationInflammationSalmonella typhimuriumAnimalsColitisDisease Models, AnimalFemaleGastrointestinal MicrobiomeHumansMiceMice, Inbred C57BLPolyethylene GlycolsPolyethylene Glycolsbowel preparationDSSgut microbiotagut-on-a-chiphumanized miceIBDIBD mouse modelinflammatory bowel diseaseosmolalitypathobiontspathogen colonizationPEGpolyethylene glycolSalmonella enterica serovar Typhimuriumtranslocation

Identifiers

PMID41435832
PMCPMC12866094

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.