ArticleBioTechniques
Real-time quantification of soluble tartrate-resistant acid phosphatase as a measure of osteoclastogenesis.
Article in BioTechniques. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed.
- Pilot Case Series of Lateral Ridge Augmentation Using a Collagenated Porcine-Derived Xenograft: Clinical, Histological, and Remodeling Outcomes.Journal of clinical medicine · 2026Article
- Real-time quantification of soluble tartrate-resistant acid phosphatase as a measure of osteoclastogenesis.BioTechniquesArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
Differentiation of osteoclasts from their macrophage precursors can be assessed via multiple methods including microscopy, gene expression analysis, and protein immunoblotting, but these methods can be expensive or labor intensive and subject to variation in approach and interpretation. We have developed a low-cost kinetic assay for the quantification of Tartrate Resistant Acid Phosphatase (TRAP), which is secreted by osteoclasts in increasing amounts as they differentiate. This assay demonstrates reliable reproducibility and provides sensitive, quantified data that accurately represents altered levels of osteoclastogenesis due to variations in Receptor Activator of Nuclear Factor κB (RANK) signaling and the presence of known osteoclast inhibitors. The assay is cost-effective, scalable and readily adoptable by labs conducting osteoclast biology research.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.