Evidence map›Paper›PMID 41432156›Full record

ArticlemSystems2026

Genomic insights into a versatile deep-sea methanotroph constituting the rare biosphere of a Brazilian carbonate mound complex.

Ana Carolina de Araújo Butarelli, Fernanda Mancini Nakamura, Francielli Vilela Peres, Flúvio Modolon da Silva, Amanda Gonçalves Bendia, Raissa Basti, Michel Michaelovitch de Mahiques, Paulo Yukio Gomes Sumida, Vivian Helena Pellizari

Abstract read
In one paragraph

Article in mSystems, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Ana Carolina de Araújo ButarelliOceanographic Institute, University of São Paulo, São Paulo, Brazil.ORCID 0000-0003-4275-492X
Fernanda Mancini NakamuraOceanographic Institute, University of São Paulo, São Paulo, Brazil.ORCID 0000-0001-6294-8604
Francielli Vilela PeresOceanographic Institute, University of São Paulo, São Paulo, Brazil.ORCID 0000-0002-5905-9168
Flúvio Modolon da SilvaOceanographic Institute, University of São Paulo, São Paulo, Brazil.ORCID 0000-0002-3671-7869
Amanda Gonçalves BendiaOceanographic Institute, University of São Paulo, São Paulo, Brazil.ORCID 0000-0003-0042-8990
Raissa BastiOceanographic Institute, University of São Paulo, São Paulo, Brazil.
Michel Michaelovitch de MahiquesOceanographic Institute, University of São Paulo, São Paulo, Brazil.
Paulo Yukio Gomes SumidaOceanographic Institute, University of São Paulo, São Paulo, Brazil.
Vivian Helena PellizariOceanographic Institute, University of São Paulo, São Paulo, Brazil.ORCID 0000-0003-2757-6352

Funding

Universidade de São Paulo in partnership with Shell Brasil and the Brazilian National Agency of Petroleum, Natural Gas and Biofuels (ANP) ANP 21012-0
6 · The paper itself

Abstract

Recent discoveries of aerobic methanotrophs in non-seep carbonate-rich environments in the deep sea suggest that these organisms may persist as part of the rare biosphere. Recovering rare, active methanotrophs through targeted culturing is essential for understanding their persistence under the oligotrophic non-seep conditions and for uncovering their genomic adaptations related to the survival in energy-limited ecosystems. In our study, using metagenomic analysis of enrichment cultures from the Alpha Crucis Carbonate Ridge, we discovered IMPORTANCE: Microbial communities in deep-sea sediments play crucial roles in global biogeochemical cycles, yet they remain poorly characterized due to the challenges of sampling and culturing under extreme conditions. This study provides a comprehensive overview of microbial diversity and functional potential in carbonate-rich deep-sea sediments, with an emphasis on methane-oxidizing bacteria. By combining high-throughput metagenomics and comparative genomics, we reconstructed high-quality genomes from previously uncharacterized microbial consortia, including novel members of the genus

Indexed as

CarbonatesSeawaterBrazilEcosystemGenome, BacterialGeologic SedimentsMetagenomicsMethanePhylogenyCarbonatesMethanedeep-sea methanotrophsgenome-resolved metagenomicsmethanotrophs cultivationMethylotuvimicrobiummicrobial consortia

Identifiers

PMID41432156
PMCPMC12911358

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.