ArticleSmall (Weinheim an der Bergstrasse, Germany)2026
Ultra-Fast Isothermal Formation of DNA Nanostructures in Culture Media: Application to In Situ Assembly of DNA Origami With Living Cells.
Article in Small (Weinheim an der Bergstrasse, Germany), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
3 citing papers in PubMed.
- DNA Nanotechnology in the Undergraduate Laboratory: Isothermal Assembly and Quantification of DNA Nanostructures.JACS Au · 2026Article
- Paranemic Cohesion of DNA under Isothermal Conditions.JACS Au · 2026Article
- Ultra-Fast Isothermal Formation of DNA Nanostructures in Culture Media: Application to In Situ Assembly of DNA Origami With Living Cells.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
Synthetic DNA strands are programmable and biocompatible building blocks that can be combined through hybridization to form user-defined nanostructures, but their assembly traditionally requires cell-incompatible conditions, imposing a lengthy ex situ fabrication step before any application with living matter. Here we demonstrate for the first time that 2D and 3D DNA origami structures can isothermally self-assemble at 37°C within minutes, directly in cell culture media, both in the absence and in the presence of living cells. Scaffold-free structures of extended dimensions, such as micrometer-long DNA nanotubes, can also self-assemble when the system is given more time to evolve. With human cell lines, 2D and 3D origami structures in situ self-assemble in 5 to 15 min, and remain stable for about 24 h and up to 3 days when actin monomers are added. Similar self-assembly performance is observed in the presence of more complex tissue-like systems, such as human induced pluripotent stem cells evolving into cerebral organoids. This ultra-fast, life-compatible self-assembly method drastically simplifies the fabrication of complex DNA nanostructures and enables the creation of in situ self-assembling nanomachines for direct and adaptive interactions with living cells.
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Registered trials
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