ArticleMicrobiologyOpen2025
Phylogenetic Footprints of Coagulase-Negative Staphylococci and Mammaliicoccus Isolated From Raw Caprine Milk.
Article in MicrobiologyOpen, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
2 citing papers in PubMed.
- Screening Equine Skin and Mucous Membranes for Carriage of Coagulase-Positive Staphylococci.Veterinary dermatology · 2026Article
- Phylogenetic Footprints of Coagulase-Negative Staphylococci and Mammaliicoccus Isolated From Raw Caprine Milk.MicrobiologyOpen · 2025Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Coagulase-negative staphylococci (CNS) and Mammaliicoccus species recently reclassified from the Staphylococcus sciuri group, are increasingly recognized as opportunistic pathogens in dairy animals and humans. This study investigated phylogenetic diversity and antimicrobial resistance in raw caprine milk from Sudan by integrating conventional bacteriological methods, molecular sequencing, and antimicrobial susceptibility testing. Raw goat milk samples were cultured, and presumptive CNS isolates were identified using phenotypic tests (novobiocin, oxidase, urease, and carbohydrate fermentation) following the standard Staphylococcus identification flow chart. PCR amplification of the elongation factor Tu (tuf) gene and the methicillin-resistance gene (mecA) enabled molecular confirmation and assessment of antimicrobial resistance. Sequenced tuf amplicons (~370 bp) were analyzed by BLAST and aligned in MEGA 12 for maximum-likelihood phylogenetic reconstruction. Staphylococcus simulans and Mammaliicoccus lentus were isolated in this study; antimicrobial susceptibility testing revealed that S. simulans, but not M. lentus, was methicillin-resistant and carried the mecA gene. Partial tuf gene sequencing confirmed 99.6%-99.7% identity with reference strains of the respective species. Phylogenetic analysis revealed that the isolated S. simulans formed a distinct branch within the global clusters. Meanwhile, M. lentus was found to be closely related to the global strains, showing only minor divergence. This study reports the presence of S. simulans and M. lentus in caprine milk from Sudan using both phenotypic and genotypic identification methods. This underscores the importance of integrating traditional laboratory methods with molecular techniques for precise species identification. The identification of methicillin-resistant S. simulans highlights the need for ongoing monitoring of CNS in raw milk.
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