Evidence map›Paper›PMID 41430715›Full record

ArticleJournal of nanobiotechnology2025

Acellular fishbone scaffolds loaded with bone marrow mesenchymal stem cell-derived exosomes for bone defect repairing.

Lei Zhu, Ji Wang, Zhengwei Liu, Kai Chen, Zhuhao Wu, Yongxiang Wang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of nanobiotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Lei ZhuDepartment of Orthopedics, Northern Jiangsu People's Hospital, Clinical Teaching Hospital of Medical School, Nanjing University, 225001, Yangzhou, China.
Ji WangDepartment of Rheumatology and Immunology, Nanjing Drum Tower Hospital, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, China.
Zhengwei LiuDepartment of Orthopedics, Northern Jiangsu People's Hospital, Clinical Teaching Hospital of Medical School, Nanjing University, 225001, Yangzhou, China.
Kai ChenDepartment of Orthopedics, Shanghai Changhai Hospital, Naval Medical University, Shanghai, 200433, China. ch_kai@163.com.
Zhuhao WuDepartment of Rheumatology and Immunology, Nanjing Drum Tower Hospital, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, China. wuzhuhao@whu.edu.cn.
Yongxiang WangDepartment of Orthopedics, Northern Jiangsu People's Hospital, Clinical Teaching Hospital of Medical School, Nanjing University, 225001, Yangzhou, China. wangyongxiang@nju.edu.cn.

Funding

Jiangsu Province Frontier Technology Research and Development Program BF2024044National Key Research and Development Program of China 2023YFB3810204National Natural Science Foundation of China 82072423Yangzhou Science and Technology Plan YZ2023266
6 · The paper itself

Abstract

Tissue engineering scaffolds remain pivotal in bone defect repair. Contemporary research in this field predominantly focuses on enhancing bone regeneration by optimizing scaffold composition and structure, and incorporating bioactive components. Herein, we developed a decellularized fish bone (DFB) scaffold integrated with hypoxia-osteogenic exosomes (HO-Exos), derived from rat bone marrow mesenchymal stem cells (BMSCs) cultured under 5% oxygen tension with 7-day osteogenic priming, to promote osseous repair via their synergistic effect. The hierarchical porosity of DFB scaffolds creates a biomimetic microenvironment conducive to BMSC adhesion and osteogenic mineralization. Functionally, HO-Exos stimulate osteogenic differentiation of BMSCs via modulation of the Wnt/β-catenin pathway, enhance cellular migration, and promote tubulogenesis in human umbilical vein endothelial cells (HUVECs). These vesicles synergistically potentiate vascularized bone regeneration in vivo through coordinated osteogenic-angiogenic regulation. To achieve sustained therapeutic delivery, HO-Exos were encapsulated within DFB scaffolds, leveraging their structural network for tailored release kinetics. In a rat model of critical-sized femoral metaphyseal defects, the designed scaffolds exhibited significant improvements in both neovascularization density and bone volume fraction compared to controls. These findings underscore the potential of exosome-functionalized biomimetic scaffolds for treating bone defects.

Indexed as

Bone RegenerationExosomesMesenchymal Stem CellsTissue ScaffoldsAnimalsBone and BonesCell DifferentiationFishesHumansHuman Umbilical Vein Endothelial CellsMaleNeovascularization, PhysiologicOsteogenesisRatsRats, Sprague-DawleyTissue EngineeringAngiogenesis enhancementBone regenerationDecellularized fish boneExosomesTissue engineering scaffolds

Identifiers

PMID41430715
PMCPMC12752130

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.