Evidence map›Paper›PMID 41430704›Full record

ArticleGenome biology2025

Comparative assessment of SNP genotyping assays for challenging forensic samples utilizing ancient DNA methods.

Adam Staadig, Maja Krzewińska, Maja Sidstedt, Daniel Kling, Siri Aili Fagerholm, Ricky Ansell, Anders Götherström, Andreas Tillmar

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Genome biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1citing papers in PubMed
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1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

8 authors.

Adam StaadigDepartment of Forensic Genetics and Forensic Toxicology, National Board of Forensic Medicine, Artillerigatan 12, Linköping, SE-587 58, Sweden. adam.staadig@liu.se.
Maja KrzewińskaCentre for Palaeogenetics, Svante Arrhenius väg 20C, Stockholm, SE-106 91, Sweden.
Maja SidstedtNational Forensic Centre, Swedish Police Authority, Linköping, SE-581 94, Sweden.
Daniel KlingDepartment of Forensic Genetics and Forensic Toxicology, National Board of Forensic Medicine, Artillerigatan 12, Linköping, SE-587 58, Sweden.
Siri Aili FagerholmNational Forensic Centre, Swedish Police Authority, Linköping, SE-581 94, Sweden.
Ricky AnsellNational Forensic Centre, Swedish Police Authority, Linköping, SE-581 94, Sweden.
Anders GötherströmCentre for Palaeogenetics, Svante Arrhenius väg 20C, Stockholm, SE-106 91, Sweden.
Andreas TillmarDepartment of Forensic Genetics and Forensic Toxicology, National Board of Forensic Medicine, Artillerigatan 12, Linköping, SE-587 58, Sweden.

Funding

The Swedish Research Council 2019-00849
6 · The paper itself

Abstract

backgroundThe fields of ancient DNA research and forensic genetics share both methodological similarities and common challenges, particularly in the analysis of degraded DNA. Leveraging these overlaps, this study evaluates three single nucleotide polymorphisms (SNP)-based genotyping assays for analyzing challenging forensic samples: the FORCE-QIAseq SNP panel, the Twist ancient DNA hybridization capture panel, and whole-genome sequencing.

resultsWe analyze twenty skeletal bone and tooth samples from authentic missing person cases, where almost all samples are severely degraded and contain exceptionally low amounts of endogenous DNA, reflected by both reduced quantifiable DNA concentrations and lower proportions of human DNA reads than typically obtained from high-quality forensic samples. Despite these challenging sample characteristics, both the FORCE and Twist assays successfully generate a substantial number of genotypes across many samples, while whole-genome sequencing yields fewer SNP calls. However, techniques like probabilistic genotyping, increase sequencing depth or genotype imputation can further enhance the utility of WGS for forensic use.

conclusionsThis study highlights the effectiveness of incorporating ancient DNA methods into forensic genetics for the analysis of degraded samples. The findings are broadly applicable to both forensic and ancient DNA research disciplines, offering valuable insights into assay selection based on sample condition and investigative goals.

Indexed as

DNA, AncientForensic GeneticsGenotyping TechniquesPolymorphism, Single NucleotideGenotypeHumansWhole Genome SequencingDNA, AncientAncient DNABoneDegraded DNAForensic geneticsForensic investigative genetic genealogyHybridization captureMassively parallel sequencingSNPWhole-genome sequencing

Identifiers

PMID41430704
PMCPMC12723910

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.