Evidence map›Paper›PMID 41430668›Full record

ArticleBMC veterinary research2025

Antigenicity analysis of the recombinant fusion proteins NlpI of Mannheimia haemolytica and DsbA of Pasteurella multocida.

Shuyun Xu, Yingni Sun, Jingjing Wei, Zengke Wei, Jie Yu, Meiqi Jiang, Hui Zhang, Zhihua Sun, Zhen Wang, Zijie Wang and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC veterinary research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Shuyun XuCollege of Animal Science and Technology, Shihezi University, Shihezi, 832003, Xinjiang, China.
Yingni SunSydney School of Veterinary Science, The University of Sydney, Camperdown, Australia.
Jingjing WeiCollege of Animal Science and Technology, Shihezi University, Shihezi, 832003, Xinjiang, China.
Zengke WeiCollege of Animal Science and Technology, Shihezi University, Shihezi, 832003, Xinjiang, China.
Jie YuCollege of Animal Science and Technology, Shihezi University, Shihezi, 832003, Xinjiang, China.
Meiqi JiangCollege of Animal Science and Technology, Shihezi University, Shihezi, 832003, Xinjiang, China.
Hui ZhangCollege of Animal Science and Technology, Shihezi University, Shihezi, 832003, Xinjiang, China.
Zhihua SunCollege of Animal Science and Technology, Shihezi University, Shihezi, 832003, Xinjiang, China.
Zhen WangCollege of Animal Science and Technology, Shihezi University, Shihezi, 832003, Xinjiang, China.
Zijie WangCollege of Animal Science and Technology, Shihezi University, Shihezi, 832003, Xinjiang, China.
Jie WuXinjiang Production and Construction Corps Eighth Division of Animal Husbandry and Veterinary Workstation, Shihezi, 832003, Xinjiang, China. 18997888015@163.com.
Xia ZhouCollege of Animal Science and Technology, Shihezi University, Shihezi, 832003, Xinjiang, China. zhoux993@163.com.

Funding

Corps Eighth Division Key Areas of Science and Technology Research Projects 2023NY03-1Corps Key Areas of Science and Technology Research 2024AB035
6 · The paper itself

Abstract

To analyze the antigenic characteristics of the recombinant fusion proteins rNlpI-DsbA (rND) of bovine Mannheimia haemolytica and Pasteurella multocida, respectively, and lay a foundation for the development of bivalent subunit vaccine development. The recombinant rND protein was successfully expressed and used to immunize mice with white oil, propolis, or astragalus polysaccharide adjuvants, respectively. Protective efficacy was evaluated by challenging mice with 5×LD₅₀ of WT M. haemolytica (Mh95) or WT P. multocida (PmA1). The rND–white oil group showed nearly 90% protection against Mh95 and 60% against PmA1, while all PBS controls died within 7 days. No significantly pathological damage was observed in the white oil group after challenge, whereas the other adjuvant groups exhibited tissue lesions following PmA1 infection. Both white oil and propolis adjuvants significantly enhanced antigen-specific IFN-γ responses and IgG titers compared to PBS controls. These results indicate that rND with white oil adjuvant induces strong immune protection and represents a promising cross-protective vaccine candidate. The rND exhibited favorable reactogenicity and immunogenicity and effectively prevented the invasion of homotypic strains. The study’s results laid a foundation for developing subunit vaccines against M. haemolytica and P. multocida.

Indexed as

Antigens, BacterialBacterial ProteinsBacterial VaccinesMannheimia haemolyticaPasteurella multocidaAdjuvants, ImmunologicAnimalsAntibodies, BacterialCattleFemaleMiceMice, Inbred BALB CPasteurella InfectionsProtein Subunit VaccinesRecombinant Fusion ProteinsAdjuvants, ImmunologicAntibodies, BacterialAntigens, BacterialBacterial ProteinsBacterial VaccinesProtein Subunit VaccinesRecombinant Fusion ProteinsDsbAImmune protectionMannheimia haemolyticaNlpIPasteurella multocidaProkaryotic expressionReactivity

Identifiers

PMID41430668
PMCPMC12870107

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.