ArticleBMC infectious diseases2025
Microbiological and molecular profiling of human hydatid cysts reveals high frequency of Klebsiella species.
Article in BMC infectious diseases, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
Abstract
backgroundHuman cystic echinococcosis (CE), caused by the metacestodes of Echinococcus granulosus, is a significant zoonotic helminth infection with medical and economic concerns in endemic regions. The metacestode, known as hydatid cyst, is frequently populated by some bacterial species. Our knowledge is poor on the nature and significance of interplay between the bacteria and hydatid cysts. This study aimed to investigate the presence of bacterial species in human hydatid cyst.
methodsA total of 101 human hydatid cyst samples isolated from liver and lungs, were collected and examined using both bacteriological and molecular techniques. The cyst materials were primarily cultured in thioglycolate medium with subsequent specific bacteriological methods of identification including bacterial culture and differential biochemical examinations. Molecular identification was performed through bacterial 16 S rDNA PCR with pan-specific primers. Also, PCR-sequencing on partial mitochondrial cox1 gene was used for molecular identification of E. granulosus isolates. The K1, K2, and RMPA primers were utilized to identify hypervirulent strains of Klebsiella pneumoniae.
resultsThe results demonstrated that 7 out of 101 samples (6.9%) were tested positive for bacteria. The most frequent bacteria found were Klebsiella species including five K. pneumoniae, one K. oxytoca and one Staphylococcus aureus. Notably two hypervirulent variants of K. pneumoniae were detected. Molecular analysis of the metacestodes indicated the presence of Echinococcus granulosus G1 and E. canadensis G6 genotypes.
conclusionOur study highlighted K. pneumoniae as a common bacterium in hydatid cysts. Further in-depth studies are warranted on the potential association between bacteria and the pathogenesis of E. granulosus. CLINICAL TRIAL NUMBER: Not applicable.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.