Evidence map›Paper›PMID 41430572›Full record

ArticleBMC genomic data2025

Unveiling complete genome sequence of Streptomyces griseoincarnatus AR2 from Katpana cold desert: the roof of the world surrounded by Karakoram mountain range.

Arisha Rameen Bhatti, Noor Hassan, Hazrat Ali, Noor Ul Huda, Habib Ur Rahman, Javeria Anwar, Anam Saqib, Mohsin Khan, Aiman Umar

Abstract read
In one paragraph

Article in BMC genomic data, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Arisha Rameen BhattiFermentation Biotechnology for Industrial Products Group, Industrial Biotechnology Division, National Institute for Biotechnology and Genetic Engineering-College, Pakistan Institute of Engineering and Applied Sciences (NIBGE-C, PIEAS), Faisalabad, Pakistan.
Noor HassanFermentation Biotechnology for Industrial Products Group, Industrial Biotechnology Division, National Institute for Biotechnology and Genetic Engineering-College, Pakistan Institute of Engineering and Applied Sciences (NIBGE-C, PIEAS), Faisalabad, Pakistan. noorhassan672@gmail.com.
Hazrat AliFermentation Biotechnology for Industrial Products Group, Industrial Biotechnology Division, National Institute for Biotechnology and Genetic Engineering-College, Pakistan Institute of Engineering and Applied Sciences (NIBGE-C, PIEAS), Faisalabad, Pakistan.
Noor Ul HudaFermentation Biotechnology for Industrial Products Group, Industrial Biotechnology Division, National Institute for Biotechnology and Genetic Engineering-College, Pakistan Institute of Engineering and Applied Sciences (NIBGE-C, PIEAS), Faisalabad, Pakistan.
Habib Ur RahmanFermentation Biotechnology for Industrial Products Group, Industrial Biotechnology Division, National Institute for Biotechnology and Genetic Engineering-College, Pakistan Institute of Engineering and Applied Sciences (NIBGE-C, PIEAS), Faisalabad, Pakistan.
Javeria AnwarFermentation Biotechnology for Industrial Products Group, Industrial Biotechnology Division, National Institute for Biotechnology and Genetic Engineering-College, Pakistan Institute of Engineering and Applied Sciences (NIBGE-C, PIEAS), Faisalabad, Pakistan.
Anam SaqibFermentation Biotechnology for Industrial Products Group, Industrial Biotechnology Division, National Institute for Biotechnology and Genetic Engineering-College, Pakistan Institute of Engineering and Applied Sciences (NIBGE-C, PIEAS), Faisalabad, Pakistan.
Mohsin KhanDepartment of Biological Sciences, Ohio University, Athens, USA.
Aiman UmarDepartment of Food and Nutrition, Faculty of Sciences, Shaheed Benazir Bhutto Women University Peshawar, Peshawar, Pakistan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectivesThe genus Streptomyces encompasses a wide range of Gram-positive bacteria, holding significant biotechnological potential. Streptomyces species have been recognized for prolific production of multiple bioactive secondary metabolites including pigments, manifesting therapeutic potential and industrial applications. However, bioactive potential of secondary metabolites produced by Streptomyces griseoincarnatus remains relatively unexplored. In this study, we isolated a S. griseoincarnatus AR2 and generated the complete genome for exploration of biosynthetic gene clusters, hence revealing secondary metabolite potential of species. DATA DESCRIPTION: S. griseoincarnatus AR2 was isolated from Katpana Cold Desert in Skardu Valley, Gilgit-Baltistan, Pakistan. The complete genome assembly of AR2 comprised of 7,307,931 bp, one contig, G + C content of 72.39%, presenting an N50 of 7,307,931 bp and an L50 of 1. However, functional annotation via RAST server revealed 6647 coding sequences (CDS), 85 RNAs and 418 subsystems in AR2 genome. Phylogenetic analysis using Type Strain Genome Server (TYGS) revealed alignment of species AR2 with reference sequence of S. griseoincarnatus (AC# CP139576.1), with bacterial orthoANI value of 98.19% and dDDH value in range between 80.9 and 86.1%. Whereas, antiSMASH v8.0.1 highlighted the presence of 23 putative secondary metabolite biosynthetic gene clusters in the genome. The respective genome sequence of AR2 provides a promising resource for exploration of biosynthetic and metabolic pathways, secondary metabolites pathway, advancing application in pharmaceutical and industrial research.

Indexed as

Genome, BacterialStreptomycesBase CompositionDesert ClimateMultigene FamilyPhylogenySecondary MetabolismWhole Genome SequencingBiosynthetic gene clustersGenome assemblySecondary metabolitesStreptomyces griseoincarnatus

Identifiers

PMID41430572
PMCPMC12903316

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.