Evidence map›Paper›PMID 41430282›Full record

ArticleJournal of neuroinflammation2025

Vagus nerve stimulation alleviates S. aureus-induced mastitis by regulating gut microbiota S24-7-PPARγ and NF-ΚB/NLRP3 signaling in mice.

Yuhong He, Yue Zhang, Lihua Zhao, Zeming Zhou, Nier Su, Can Zhang, Keyi Wang, Lei Jin, Bin Yang, Xiaoyu Hu and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of neuroinflammation, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Yuhong He *Department of Breast Surgery, Union Hospital of Jilin University, Changchun, Jilin, 130033, China.
Yue Zhang *College of Animal Science and Technology, Jilin Agriculture Science and Technology University, Jilin City, China.
Lihua Zhao *Department of Breast Surgery, Union Hospital of Jilin University, Changchun, Jilin, 130033, China.
Zeming ZhouDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, Jilin Province, 130062, China.
Nier SuDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, Jilin Province, 130062, China.
Can ZhangDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, Jilin Province, 130062, China.
Keyi WangDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, Jilin Province, 130062, China.
Lei JinDepartment of Anesthesiology, Union Hospital of Jilin University, Changchun, Jilin, 130033, China.
Bin YangDepartment of Breast Surgery, Union Hospital of Jilin University, Changchun, Jilin, 130033, China. y_bin@jlu.edu.cn.
Xiaoyu HuDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, Jilin Province, 130062, China. huxiaoyu@jlu.edu.cn.
Yunhe FuDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, Jilin Province, 130062, China. fuyunhesky@sina.com.

Funding

Jilin Province Department of Science and Technology 212558JC010286222the National Natural Science Foundation of China 32301247the National Natural Science Foundation of China 32422086
6 · The paper itself

Abstract

Gut microbiota dysbiosis has been implicated in the pathogenesis of mastitis. While the vagus nerve exerts well-documented anti-inflammatory effects and modulates gut microbiota, its potential influence on mastitis progression via gut microbiota modulation remains unclear. To investigate this, we employed vagus nerve stimulation (VNS) in Staphylococcus aureus (S. aureus)-induced mastitis in mice. We demonstrate that VNS significantly attenuated mammary gland inflammation and restored epithelial barrier integrity following S. aureus challenge. Crucially, antibiotic depletion of the gut microbiota abrogated the protective effects of VNS, and fecal microbiota transplantation (FMT) from VNS-treated mice conferred protection against mastitis, establishing a causal role for the gut microbiota in mediating the VNS effect. Specifically, VNS markedly increased the abundance of Muribaculaceae. in the gut. Replenishment with S24-7, a representative strain of this genus, alleviated S. aureus-induced mammary gland inflammation in mice. Transcriptomic analysis revealed that S24-7 exerted its effects by activating peroxisome proliferator-activated receptor gamma (PPARγ), which subsequently suppressed the NF-κB/NLRP3 signaling pathway. Overall, our findings suggest that targeting the vagus nerve - mediated Muribaculaceae/PPARγ axis may represent a promising strategy for mastitis treatment.

Indexed as

Gastrointestinal MicrobiomeMastitisStaphylococcal InfectionsVagus Nerve StimulationAnimalsFemaleMiceMice, Inbred BALB CNF-kappa BNLR Family, Pyrin Domain-Containing 3 ProteinPPAR gammaSignal TransductionStaphylococcus aureusNF-kappa BNLR Family, Pyrin Domain-Containing 3 ProteinNlrp3 protein, mousePPAR gammaPparg protein, mouseGut microbiotaMastitisPPARγ/NF-ΚB/NLRP3S24-7S.aureusVNS

Identifiers

PMID41430282
PMCPMC12838515

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.