Evidence map›Paper›PMID 41430075›Full record

ArticleNature communications2025

Afterglow ice formed by phosphorescent luminophore-protein conjugates and complexes in aqueous solution at freezing temperature.

Xun Li, Jiuyang Li, Guangming Wang, Yuming Su, Minjian Wu, Kaka Zhang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Xun LiState Key Laboratory of Organometallic Chemistry and Shanghai Hongkong Joint Laboratory in Chemical Synthesis, Key Laboratory of Synthetic and Self-Assembly Chemistry for Organic Functional Molecules, Shanghai Institute of Organic Chemistry, University of Chinese Academy of Sciences, Chinese Academy of Sciences, 345 Lingling Road, Shanghai, 200032, People's Republic of China.
Jiuyang LiState Key Laboratory of Organometallic Chemistry and Shanghai Hongkong Joint Laboratory in Chemical Synthesis, Key Laboratory of Synthetic and Self-Assembly Chemistry for Organic Functional Molecules, Shanghai Institute of Organic Chemistry, University of Chinese Academy of Sciences, Chinese Academy of Sciences, 345 Lingling Road, Shanghai, 200032, People's Republic of China.
Guangming WangState Key Laboratory of Organometallic Chemistry and Shanghai Hongkong Joint Laboratory in Chemical Synthesis, Key Laboratory of Synthetic and Self-Assembly Chemistry for Organic Functional Molecules, Shanghai Institute of Organic Chemistry, University of Chinese Academy of Sciences, Chinese Academy of Sciences, 345 Lingling Road, Shanghai, 200032, People's Republic of China.
Yuming SuState Key Laboratory of Organometallic Chemistry and Shanghai Hongkong Joint Laboratory in Chemical Synthesis, Key Laboratory of Synthetic and Self-Assembly Chemistry for Organic Functional Molecules, Shanghai Institute of Organic Chemistry, University of Chinese Academy of Sciences, Chinese Academy of Sciences, 345 Lingling Road, Shanghai, 200032, People's Republic of China.
Minjian WuState Key Laboratory of Organometallic Chemistry and Shanghai Hongkong Joint Laboratory in Chemical Synthesis, Key Laboratory of Synthetic and Self-Assembly Chemistry for Organic Functional Molecules, Shanghai Institute of Organic Chemistry, University of Chinese Academy of Sciences, Chinese Academy of Sciences, 345 Lingling Road, Shanghai, 200032, People's Republic of China.
Kaka ZhangState Key Laboratory of Organometallic Chemistry and Shanghai Hongkong Joint Laboratory in Chemical Synthesis, Key Laboratory of Synthetic and Self-Assembly Chemistry for Organic Functional Molecules, Shanghai Institute of Organic Chemistry, University of Chinese Academy of Sciences, Chinese Academy of Sciences, 345 Lingling Road, Shanghai, 200032, People's Republic of China. zhangkaka@sioc.ac.cn.ORCID http://orcid.org/0000-0002-8176-6205

Funding

National Natural Science Foundation of China (National Science Foundation of China) 22475228, 22175194Natural Science Foundation of Ningbo (Ningbo Natural Science Foundation) 2023J243
6 · The paper itself

Abstract

Unlike the widely reported fluorophore-biomacromolecule systems in nucleic acid testing and immunoassay, organic afterglow luminophore-biomacromolecule conjugates and complexes remain rarely explored. Here we report the observation of organic afterglow from aqueous solutions of luminophore-protein conjugates and complexes at freezing temperature, named as afterglow ice for abbreviation. Reliable N-hydroxysuccinimide ester protocol, as well as β-diketone chemistry, are applied for protein labeling to form luminophore-protein conjugates, which exhibit intriguing afterglow at freezing temperature. Control experiments reveal that hydrophobic interaction and covalent linkage between luminophore and protein can protect organic triplet excited states from quenching. In the case of luminophore-protein complex, we observe the switching-on of organic afterglow after specific recognition of streptavidin by biotinylated luminophore, which is the example of specific recognition and sensing of biomacromolecules by organic afterglow emitters. Although it is not yet a mature technology for biomedical applications, this study represents the initial step of organic afterglow materials towards bio-labeling and bioassay fields, as well as expanding the application scenarios of bioactive products.

Indexed as

Fluorescent DyesFreezingIceLuminescent AgentsProteinsHydrophobic and Hydrophilic InteractionsSolutionsStreptavidinSuccinimidesTemperatureWaterFluorescent DyesIceLuminescent AgentsProteinsSolutionsStreptavidinSuccinimidesWater

Identifiers

PMID41430075
PMCPMC12779962

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.