Evidence map›Paper›PMID 41428865›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2025

Antibiotic-induced microbiota depletion impairs the proregenerative response to a biological scaffold.

Natalie Rutkowski, Brenda Yang, Elise Gray-Gaillard, Anna Ruta, Joscelyn C Mejías, Michael Patatanian, Christopher Cherry, Nazmiye Celik, Katlin B Stivers, Shri Ramanujam and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Antibiotic-induced microbiota depletion impairs the proregenerative response to a biological scaffold.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

15 authors.

Natalie RutkowskiDepartment of Biomedical Engineering, Cellular and Molecular Medicine, or Ophthalmology, Translational Tissue Engineering Center, Johns Hopkins University, Baltimore, MD 21231.
Brenda YangDepartment of Biomedical Engineering, Cellular and Molecular Medicine, or Ophthalmology, Translational Tissue Engineering Center, Johns Hopkins University, Baltimore, MD 21231.
Elise Gray-GaillardDepartment of Biomedical Engineering, Cellular and Molecular Medicine, or Ophthalmology, Translational Tissue Engineering Center, Johns Hopkins University, Baltimore, MD 21231.
Anna RutaDepartment of Biomedical Engineering, Cellular and Molecular Medicine, or Ophthalmology, Translational Tissue Engineering Center, Johns Hopkins University, Baltimore, MD 21231.
Joscelyn C MejíasDepartment of Biomedical Engineering, Cellular and Molecular Medicine, or Ophthalmology, Translational Tissue Engineering Center, Johns Hopkins University, Baltimore, MD 21231.
Michael PatatanianDepartment of Biomedical Engineering, Cellular and Molecular Medicine, or Ophthalmology, Translational Tissue Engineering Center, Johns Hopkins University, Baltimore, MD 21231.
Christopher CherryDepartment of Biomedical Engineering, Cellular and Molecular Medicine, or Ophthalmology, Translational Tissue Engineering Center, Johns Hopkins University, Baltimore, MD 21231.
Nazmiye CelikDepartment of Biomedical Engineering, Cellular and Molecular Medicine, or Ophthalmology, Translational Tissue Engineering Center, Johns Hopkins University, Baltimore, MD 21231.
Katlin B StiversDepartment of Biomedical Engineering, Cellular and Molecular Medicine, or Ophthalmology, Translational Tissue Engineering Center, Johns Hopkins University, Baltimore, MD 21231.
Shri RamanujamDepartment of Biomedical Engineering, Cellular and Molecular Medicine, or Ophthalmology, Translational Tissue Engineering Center, Johns Hopkins University, Baltimore, MD 21231.
Nathan L PriceTranslational Gerontology Branch, National Institute on Aging, Baltimore, MD 21224.
Franck HousseauDepartment of Oncology and Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD 21287.ORCID 0000-0002-7028-3953
Drew M PardollDepartment of Oncology and Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD 21287.ORCID 0000-0001-6215-1013
Cynthia L SearsDepartment of Oncology and Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD 21287.ORCID 0000-0003-4059-1661
Jennifer H ElisseeffDepartment of Biomedical Engineering, Cellular and Molecular Medicine, or Ophthalmology, Translational Tissue Engineering Center, Johns Hopkins University, Baltimore, MD 21231.

Funding

Age and sex differences in the immune response to synthetic materialsK99AG081564 · NIA · JOHNS HOPKINS UNIVERSITY · PI MEJIAS, JOSCELYN CLARALUZ · 2023 to 2024
$202k
NIH HHS K99AG081564NSF | NSF Graduate Research Fellowship Program (GRFP) DGE1746891NSF | NSF Graduate Research Fellowship Program (GRFP) DGE2139757
6 · The paper itself

Abstract

Therapeutic biological scaffolds promote tissue repair primarily through the induction of type 2 immunity. However, systemic immunological factors, including aging, sex, and previous infections, can modulate this response. The gut microbiota is a well-established modulator of immune function across organ systems, yet its influence on type 2-mediated repair remains underexplored. Here, we establish a bidirectional relationship between the gut microbiota and biological scaffold-mediated tissue repair. Utilizing a conventionalized germ-free mouse, we demonstrate that scaffold implantation induces compositional and functional changes in the gut microbiome, particularly affecting amino acid biosynthesis. Additionally, in a model of antibiotic-induced microbiota depletion, we show that dysbiosis disrupts key immune regulators of type 2 immunity, including reductions in eosinophils, proregenerative macrophages, and interleukin-4 (IL-4)-producing CD4

Indexed as

Anti-Bacterial AgentsGastrointestinal MicrobiomeTissue ScaffoldsAnimalsCD4-Positive T-LymphocytesDysbiosisFemaleInterleukin-4MacrophagesMaleMiceMice, Inbred C57BLAnti-Bacterial AgentsInterleukin-4antibioticbiomaterialsgut microbiotaimmune responsetissue repair

Identifiers

PMID41428865
PMCPMC12772165

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.