Evidence map›Paper›PMID 41428486›Full record

ArticleCell reports2026

Targeted gene transfer into developmentally defined cell populations of the primate brain.

Ana Rita Ribeiro Gomes, Natalie Hamel, Surjeet Mastwal, Naim Wright, David C Ide, Christopher T Richie, Ted B Usdin, Kuan Hong Wang, David A Leopold

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors.

Ana Rita Ribeiro GomesSection on Cognitive Neurophysiology and Imaging, Systems Neurodevelopment Laboratory, National Institute of Mental Health, National Institutes of Health, Bethesda, MD, USA. Electronic address: anarita.ribeirogomes@nih.gov.
Natalie HamelSection on Cognitive Neurophysiology and Imaging, Systems Neurodevelopment Laboratory, National Institute of Mental Health, National Institutes of Health, Bethesda, MD, USA.
Surjeet MastwalSection on Cognitive Neurophysiology and Imaging, Systems Neurodevelopment Laboratory, National Institute of Mental Health, National Institutes of Health, Bethesda, MD, USA.
Naim WrightSection on Cognitive Neurophysiology and Imaging, Systems Neurodevelopment Laboratory, National Institute of Mental Health, National Institutes of Health, Bethesda, MD, USA.
David C IdeSection on Instrumentation, National Institute of Mental Health, National Institutes of Health, Bethesda, MD, USA.
Christopher T RichieGenetic Engineering and Viral Vector Core, National Institute of Drug Abuse, National Institutes of Health, Baltimore, MD, USA.
Ted B UsdinSystems Neuroscience Imaging Resource, National Institute of Mental Health, National Institutes of Health, Bethesda, MD, USA.
Kuan Hong WangDepartment of Neuroscience, Del Monte Institute for Neuroscience, University of Rochester Medical Center, Rochester, NY, USA. Electronic address: kuanhong_wang@urmc.rochester.edu.
David A LeopoldSection on Cognitive Neurophysiology and Imaging, Systems Neurodevelopment Laboratory, National Institute of Mental Health, National Institutes of Health, Bethesda, MD, USA; National Institute for Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD, USA. Electronic address: leopoldd@mail.nih.gov.

Funding

Visual Adaptation and Neuronal SelectivityZIAMH002898 · NIMH · NATIONAL INSTITUTE OF MENTAL HEALTH · PI LEOPOLD, DAVID A · 2009 to 2025
$13.6M
Intramural NIH HHS ZIA MH002898
6 · The paper itself

Abstract

The primate brain possesses unique physiological and developmental features, yet its systematic investigation has been hampered by a paucity of transgenic germline models and tools. Here, we present a minimally invasive method to introduce transgenes widely across the primate cerebral cortex using ultrasound-guided fetal intracerebroventricular viral injections (FIVIs). FIVI enables efficient and long-lasting transgene expression following intrauterine delivery of recombinant adeno-associated viruses (rAAVs). In the marmoset, we demonstrate that adjusting gestational timing, rAAV serotype, and transcriptional regulatory elements enables selective targeting of defined cell populations, including layer-restricted labeling and Cre-dependent intersectional access. Pilot experiments in rats further demonstrate the potential of FIVIs for prenatal CRISPR-based gene editing and labeling of peripheral somatosensory and retinal pathways. By mimicking key desirable features of germline transgenic models, this efficient and targeted method for gene transfer into the fetal primate brain expands the experimental opportunities for basic and translational neuroscience research across the lifespan.

Indexed as

BrainGene Transfer TechniquesAnimalsCallithrixCRISPR-Cas SystemsDependovirusFemaleGene EditingGenetic VectorsMaleRatsTransgenesadeno-associated virusCallithrix jacchuscerebral cortexCP: neurosciencefetusmarmosetneurodevelopmentneuronsnonhuman primatepregnancyprenatalprenatal gene deliveryprimateratRattus norvegicusultrasound-guided intracerebroventricular injectionsviral tropismviral vectors

Identifiers

PMID41428486
PMCPMC12969075

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.