Evidence map›Paper›PMID 41428227›Full record

ArticleMikrochimica acta2025

Low-power microfluidic RT-LAMP system with real-time fluorescence detection for portable nucleic acid testing.

Antao Sun, Petra Stejskalová, Xiang Zhen, Haiyang Lu, Xiaocheng Liu, Jan Brodský, Imrich Gablech, Ondřej Zítka, Hanliang Zhu, Pavel Neužil

Abstract read
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In one paragraph

Article in Mikrochimica acta, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Antao SunMinistry of Education Key Laboratory of Micro and Nano Systems for Aerospace, School of Mechanical Engineering, Northwestern Polytechnical University, 127 West Youyi Road, Xi'an, Shaanxi, 710072, PR China.
Petra StejskalováDepartment of Chemistry and Biochemistry, Mendel University in Brno, Zemědělská 1, Brno, 61300, Czech Republic.
Xiang ZhenMinistry of Education Key Laboratory of Micro and Nano Systems for Aerospace, School of Mechanical Engineering, Northwestern Polytechnical University, 127 West Youyi Road, Xi'an, Shaanxi, 710072, PR China.
Haiyang LuMinistry of Education Key Laboratory of Micro and Nano Systems for Aerospace, School of Mechanical Engineering, Northwestern Polytechnical University, 127 West Youyi Road, Xi'an, Shaanxi, 710072, PR China.
Xiaocheng LiuMinistry of Education Key Laboratory of Micro and Nano Systems for Aerospace, School of Mechanical Engineering, Northwestern Polytechnical University, 127 West Youyi Road, Xi'an, Shaanxi, 710072, PR China.
Jan BrodskýDepartment of Microelectronics, Faculty of Electrical Engineering and Communication, Brno University of Technology, Technická 3058/10, Brno, 61600, Czech Republic.
Imrich GablechDepartment of Microelectronics, Faculty of Electrical Engineering and Communication, Brno University of Technology, Technická 3058/10, Brno, 61600, Czech Republic.
Ondřej ZítkaDepartment of Chemistry and Biochemistry, Mendel University in Brno, Zemědělská 1, Brno, 61300, Czech Republic.
Hanliang ZhuMinistry of Education Key Laboratory of Micro and Nano Systems for Aerospace, School of Mechanical Engineering, Northwestern Polytechnical University, 127 West Youyi Road, Xi'an, Shaanxi, 710072, PR China. hanlz@mail.nwpu.edu.cn.
Pavel NeužilMinistry of Education Key Laboratory of Micro and Nano Systems for Aerospace, School of Mechanical Engineering, Northwestern Polytechnical University, 127 West Youyi Road, Xi'an, Shaanxi, 710072, PR China. pavel.neuzil@nwpu.edu.cn.

Funding

National Centre for Biotechnology in Veterinary Medicine TN02000017
6 · The paper itself

Abstract

A microfluidic reverse transcription loop-mediated isothermal amplification (RT-LAMP) platform is presented featuring integrated real-time fluorescence detection and optimized thermal control. Magnetic bead-based RNA extraction was paired with a thermally insulated reaction chip operated under vacuum to suppress heat loss. Additional energy-saving strategies, including reduced wire cross-section and low-emissivity surface treatment, reduced total power consumption from (2.00 ± 0.08) W to (0.29 ± 0.01) W, corresponding to an 85.5 % reduction. Amplification was completed within 20 min, and positive fluorescence signals were detected in less than 11 min. Although LAMP reagents remain more expensive than those used in PCR, the substantial energy savings and system simplification demonstrated here support practical battery-powered operation. The RT-LAMP chemistry used in this system matched a previously validated configuration, which achieved a limit of detection of 256 RNA copies and a signal slope of (- 1.70 ± 0.20) dec.min⁻

Indexed as

Microfluidic Analytical TechniquesMolecular Diagnostic TechniquesNucleic Acid Amplification TechniquesRNAFluorescenceLimit of DetectionRNALow-power diagnosticsMicrofluidic biosensorNucleic acid amplificationPoint-of-care testingRT-LAMP

Identifiers

PMID41428227

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.