Evidence map›Paper›PMID 41428119›Full record

ArticleMedical microbiology and immunology2025

A comprehensive multi-epitope recombinant protein strategy for accurate serological detection of Toxoplasma gondii infection.

Amirreza Javadi Mamaghani, Maryam Karimi, Ali Haghighi, Seyyed Javad Seyyed Tabaei, Omid Ali Adeli, Somayeh Aghamolaei, Maryam Ebrahimi, Shirzad Fallahi, Ali Mirzapour, Mohammad Yarahmadi

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Article in Medical microbiology and immunology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

10 authors.

Amirreza Javadi Mamaghani *Hepatitis Research Center, Department of Medical Parasitology and Mycology, Lorestan University of Medical Sciences, Khorramabad, Iran. amirdpara.beheshti@gmail.com.
Maryam KarimiCellular and Molecular Research Center, Kurdistan University of Medical Science, Sanandaj, Iran.
Ali HaghighiDepartment of Parasitology and Mycology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Seyyed Javad Seyyed Tabaei *Department of Parasitology and Mycology, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Seyyedtabaei@gmail.com.
Omid Ali AdeliDepartment of Pathology, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran.
Somayeh AghamolaeiDepartment of Parasitology and Mycology, Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
Maryam EbrahimiDepartment of Parasitology and Mycology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Shirzad FallahiHepatitis Research Center, Department of Medical Parasitology and Mycology, Lorestan University of Medical Sciences, Khorramabad, Iran.
Ali MirzapourInnovative Medical Research Center, Department of Medical Parasitology and Mycology, School of Medicine, Mashhad Branch, Islamic Azad University, Mashhad, Iran.
Mohammad YarahmadiDepartment of Medical Parasitology and Mycology, Lorestan University of Medical Sciences, Khorramabad, Iran.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Toxoplasma gondii is a globally prevalent zoonotic parasite responsible for toxoplasmosis. Accurate serological diagnosis remains challenging due to the lack of standardized, high-performance antigens. In this study, we designed a recombinant multi-epitope protein (ME-TG) by selecting B-cell epitopes from four immunodominant antigens: SAG1, GRA7, GRA4, and GRA14. The ME-TG construct was expressed in E. coli, purified, and evaluated using ELISA across 189 serum samples, including acute (n = 45), chronic (n = 38), healthy controls (n = 62), and individuals with other parasitic or bacterial infections (n = 42). The ME-TG-based ELISA demonstrated 98% sensitivity for both IgG and IgM detection, and 100% specificity, with no observed cross-reactivity. These findings highlight the diagnostic potential of ME-TG as a standardized serological marker for human toxoplasmosis. Further validation using larger, geographically diverse cohorts and additional T. gondii antigens is recommended to enhance clinical applicability.

Indexed as

Antibodies, ProtozoanAntigens, ProtozoanEpitopes, B-LymphocyteSerologic TestsToxoplasmaToxoplasmosisAdultEnzyme-Linked Immunosorbent AssayEscherichia coliFemaleHumansImmunoglobulin GImmunoglobulin MMiddle AgedProtozoan ProteinsRecombinant ProteinsAntibodies, ProtozoanAntigens, ProtozoanEpitopes, B-LymphocyteImmunoglobulin GImmunoglobulin MProtozoan ProteinsRecombinant ProteinsBioinformaticsELISAMulti-epitope recombinant proteinSerodiagnosisToxoplasma gondii

Identifiers

PMID41428119

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