Evidence map›Paper›PMID 41427733›Full record

ArticleProtein science : a publication of the Protein Society2026

Dynamic interaction of the Yersinia pseudotuberculosis type three secretion system proteins LcrV and LcrG.

Jagadish Chandra Kumar Mangu, Per Rogne, Jonna Mattsson, Lucas Hultgren, Kumar D Gahlot, Anaïs Lamy, Ronnie P-A Berntsson, Lennart B-Å Johansson, Matthew S Francis, Magnus Wolf-Watz

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In one paragraph

Article in Protein science : a publication of the Protein Society, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Jagadish Chandra Kumar ManguDepartment of Chemistry, Umeå University, Umeå, Sweden.ORCID https://orcid.org/0000-0003-1850-1412
Per RogneDepartment of Chemistry, Umeå University, Umeå, Sweden.
Jonna MattssonDepartment of Chemistry, Umeå University, Umeå, Sweden.
Lucas HultgrenBioMS, Department of Clinical Sciences, Lund University, Lund, Sweden.
Kumar D GahlotDepartment of Molecular Biology, Umeå University, Umeå, Sweden.
Anaïs LamyDepartment of Medical Biochemistry and Biophysics, Umeå University, Umeå, Sweden.
Ronnie P-A BerntssonDepartment of Medical Biochemistry and Biophysics, Umeå University, Umeå, Sweden.
Lennart B-Å JohanssonDepartment of Chemistry, Umeå University, Umeå, Sweden.
Matthew S FrancisDepartment of Molecular Biology, Umeå University, Umeå, Sweden.
Magnus Wolf-WatzDepartment of Chemistry, Umeå University, Umeå, Sweden.

Funding

Excellence by choice post-doctoral fellowship grant from Kempe foundationVetenskapsrådet 2021-04513
6 · The paper itself

Abstract

Yersinia pathogenicity is dependent on polarized translocation of effector proteins via the type III secretion system (T3SS). The tip complex situated on the needle structure of the T3SS is required for contact with the eukaryotic host membrane and is to an extent composed of pentameric LcrV. LcrV is a multifunctional protein that also acts as a regulator of the T3SS by virtue of forming a high-affinity complex in the cytoplasm with its chaperone, LcrG. By employing a structure-based approach centered on mass spectrometry, FRET and NMR spectroscopy, we demonstrated that the LcrV-LcrG complex is best described as a multivalent complex, and that the N-terminal domain of LcrV contributes by negatively affecting the LcrG binding affinity. The N-terminal domain of LcrV is dynamic and undergoes a conformational change to accommodate LcrG binding.

Indexed as

Antigens, BacterialBacterial ProteinsPore Forming Cytotoxic ProteinsType III Secretion SystemsYersinia pseudotuberculosisModels, MolecularNuclear Magnetic Resonance, BiomolecularProtein BindingProtein ConformationProtein DomainsAntigens, BacterialBacterial ProteinsLcrV protein, YersiniaPore Forming Cytotoxic ProteinsType III Secretion Systemschaperone LcrGneedle tip complexpentameric LcrVprotein conformational switchtype III secretion systemYersinia pathogenicity

Identifiers

PMID41427733
PMCPMC12720783

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.