Evidence map›Paper›PMID 41427400›Full record

ArticlebioRxiv : the preprint server for biology2025

High-Dimensional Single-Cell Analysis Reveals Coordinated Age-Dependent Neuroinflammatory Microglia-T cell Circuits in the Brain.

Md Akkas Ali, Md Hasanul Banna Siam, Donald Vardaman, Chase Bolding, J Nicholas Brazell, Ashleigh D Whatley, Christopher A Risley, Harrison Tidwell, Syed Nakib Hossain, Juhi Samal and 3 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Md Akkas AliDepartment of Pathology, Heersink School of Medicine, University of Alabama at Birmingham, Birmingham, AL, 35205, USA.
Md Hasanul Banna SiamDepartment of Pathology, Heersink School of Medicine, University of Alabama at Birmingham, Birmingham, AL, 35205, USA.
Donald VardamanDepartment of Pathology, Heersink School of Medicine, University of Alabama at Birmingham, Birmingham, AL, 35205, USA.
Chase BoldingDepartment of Pathology, Heersink School of Medicine, University of Alabama at Birmingham, Birmingham, AL, 35205, USA.
J Nicholas BrazellDepartment of Pathology, Heersink School of Medicine, University of Alabama at Birmingham, Birmingham, AL, 35205, USA.
Ashleigh D WhatleyDepartment of Pathology, Heersink School of Medicine, University of Alabama at Birmingham, Birmingham, AL, 35205, USA.
Christopher A RisleyDepartment of Microbiology, Heersink School of Medicine, University of Alabama at Birmingham, Birmingham, AL, 35233, USA.
Harrison TidwellDepartment of Pathology, Heersink School of Medicine, University of Alabama at Birmingham, Birmingham, AL, 35205, USA.
Syed Nakib HossainEuropean Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI), Wellcome Genome Campus, Hinxton, Cambridge CB10 1SD, UK.
Juhi SamalDepartment Biomedical Engineering, School of Engineering, University of Alabama at Birmingham, Birmingham, AL, 35205, USA.
Ashley S HarmsDepartment of Neurology, Heersink School of Medicine, University of Alabama at Birmingham, Birmingham, AL, 35294, USA.
Mallikarjun PatilDepartment of Pathology, Heersink School of Medicine, University of Alabama at Birmingham, Birmingham, AL, 35205, USA.
Daniel J TyrrellDepartment of Pathology, Heersink School of Medicine, University of Alabama at Birmingham, Birmingham, AL, 35205, USA.ORCID 0000-0002-0811-6724

Funding

XRAY CRYSTALLOGRAPHYP30CA013148 · NCI · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI Omer Jamy · 1985 to 2026
$165.9M
Virology CoreP30AI027767 · NIAID · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI Paul A. Goepfert · 1988 to 2026
$84.1M
Sex hormones and arthritis in a long lived animal modelP30AG050886 · NIA · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI Daniel L Smith · 2015 to 2026
$11.3M
Cerebrovascular Mitochondrial Function in Aging and Ischemic InjuryR00AG068309 · NIA · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI TYRRELL, DANIEL · 2022 to 2024
$744k
Multi-parameter, analytic flow cytometerS10OD032296 · OD · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI RANDALL, TROY D · 2022 to 2022
$464k
Cerebrovascular Mitochondrial Function in Aging and Ischemic InjuryK99AG068309 · NIA · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI TYRRELL, DANIEL · 2021 to 2022
$131k
NCI NIH HHS P30 CA013148NIAID NIH HHS P30 AI027767NIA NIH HHS K99 AG068309NIA NIH HHS P30 AG050886NIA NIH HHS R00 AG068309NIH HHS S10 OD032296
6 · The paper itself

Abstract

Aging and cerebrovascular pathology drive neuroinflammation in vascular dementia (VaD) but immune mechanisms underlying this interplay remain unresolved. Leveraging multi-modal high-dimensional imaging, flow cytometry, and split pool ligation transcriptomic sequencing in a mouse model of VaD, we constructed a brain immune cell atlas spanning young and aged mice in health and disease. We profiled microglia, T cells, macrophages, neutrophils, and B cells and integrated transcriptomics, cell-cell communication, multiplex imaging, and comparative analysis with human microglia. We found striking depletion of

Identifiers

PMID41427400
PMCPMC12712942

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.