Evidence map›Paper›PMID 41427371›Full record

ArticlebioRxiv : the preprint server for biology2025

Spatiotemporal Atlas of Heart Development Reveals Blood-Flow-Dependent Cellular, Structural, Metabolic, and Spatial Remodeling.

Jooyoung Park, Shuofei Sun, Rohit Agarwal, Andreas Stephanou, Mong Lung Steven Poon, Hyun Maeng, Peyton Lancaster, Iwijn De Vlaminck, Jonathan Butcher

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Jooyoung ParkMeinig School of Biomedical Engineering, Cornell University, Ithaca, NY, USA.ORCID 0000-0001-9001-6388
Shuofei SunMeinig School of Biomedical Engineering, Cornell University, Ithaca, NY, USA.
Rohit AgarwalMeinig School of Biomedical Engineering, Cornell University, Ithaca, NY, USA.
Andreas StephanouMeinig School of Biomedical Engineering, Cornell University, Ithaca, NY, USA.
Mong Lung Steven PoonMeinig School of Biomedical Engineering, Cornell University, Ithaca, NY, USA.
Hyun MaengMeinig School of Biomedical Engineering, Cornell University, Ithaca, NY, USA.
Peyton LancasterMeinig School of Biomedical Engineering, Cornell University, Ithaca, NY, USA.
Iwijn De VlaminckMeinig School of Biomedical Engineering, Cornell University, Ithaca, NY, USA.
Jonathan ButcherMeinig School of Biomedical Engineering, Cornell University, Ithaca, NY, USA.ORCID 0000-0002-9309-6296

Funding

Mechanobiology of Cardiac Outflow Tract MorphogenesisR01HL160028 · NHLBI · CORNELL UNIVERSITY · PI BUTCHER, JONATHAN TALBOT · 2022 to 2025
$3.1M
NHLBI NIH HHS R01 HL160028
6 · The paper itself

Abstract

Embryonic heart development depends on coordinated interactions between cellular programs, molecular signaling, and biomechanical forces, yet how mechanical cues shape cellular and molecular pathways remains incompletely understood. We perturbed cardiac blood flow by partial left or right atrial ligation (LAL/RAL) in chick embryos, generating chamber-specific hemodynamic gain- or loss-of-function states. Using single-cell and unbiased, high-resolution spatial transcriptomics, we generated a spatiotemporal atlas of flow-dependent tissue development, enabling systematic investigations of bidirectional interactions between blood-flow mechanics and tissue development. Spatially resolved analyses recapitulated key features of normal morphogenesis including regional maturation and the cellular neighborhood. We further revealed flow-specific remodeling across molecular, cellular, and architectural levels. Altered flow induced LOX-expressing cardiomyocyte and endocardial states, disrupted ventricular layer organization, and delayed maturation, alongside transient metabolic and ion-transport adaptations. Together, these findings define how redistributed blood flow reshapes developing cardiac tissues and provide a framework for studying flow-dependent remodeling in morphogenesis and malformation.

Indexed as

Cardiac morphogenesisCell NeighborhoodCongenital heart defectsHeart valveHeart VentricleHemodynamicsMesoscalePlasticitySingle-cell transcriptomicsSpatial transcriptomics

Identifiers

PMID41427371
PMCPMC12714022

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.