ArticleACS omega2025
Semisynthetic, Multicolor Probes for Bioluminescence Imaging.
Article in ACS omega, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Authors and funding
8 authors.
Funding
Abstract
Bioluminescence imaging is a sensitive and noninvasive technique for monitoring molecular and cellular processes in living systems. NanoLuc is one of the brightest and most widely used luciferase enzymes; however, the native blue emission of NanoLuc limits its applications due to poor sample penetration. NanoBiT, a split luciferase reporter derived from NanoLuc, consists of two subunits: LgBiT and HiBiT. Herein, we leverage bioluminescence resonance energy transfer (BRET) to red-shift the emission of NanoBiT by chemically labeling the N-terminus of HiBiT with fluorescent probes. Upon association of HiBiT and LgBiT, the appended fluorophores emit red-shifted light. When LgBiT is genetically encoded on the surface of mammalian cells, each probe produces unique emission profiles that can be distinguished by spectral phasor analysis microscopy. Moreover, by conjugating HiBiT peptides to fluorescent quantum dots, we extended BRET emission into the near-infrared. This modular system allows easy synthesis, rapid color switching, and tunable emission across the visible to near-infrared spectrum, providing a versatile platform for multicolor bioluminescence imaging.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.