ArticleSovremennye tekhnologii v meditsine2025
The Use of High-Concentration Collagen-Based Composition and Gelatin Granules as Bioinks for Extrusion 3D Bioprinting of Porous-Structured Hydrogel Constructs.
Article in Sovremennye tekhnologii v meditsine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Materials and Methods: Bioprinting was performed on a 3D Invivo bioprinter (Rokit, South Korea). We assessed the filament continuity during extrusion, the changes in its thickness after test printing and incubation, as well as the biodegradation of prepared scaffolds. The hydrogel cytocompatibility was studied by the proliferation of adipose-derived stem cells (ADSCs) incorporated into the scaffolds. Flow cytometry was performed to determine the immunophenotype of ADSCs. Cell proliferation in the scaffold structure was studied Results: The extrusion of the high concentration collagen-based hydrogel composition (40 mg/ml) and gelatin granules (6.25 mg/ml) during printing was stable, there was no filament breakage. When incubated in phosphate-buffered saline, the filament thickness of the hydrogel was statistically significantly higher than the scaffold thickness after printing. The degradation of the scaffolds from the hydrogel and gelatin in the solution of type I collagenase started earlier than the collagen scaffolds. The incubation in phosphate-buffered saline for 14 days resulted in less mass loss when drying the collagen scaffolds with gelatin granules. The cells isolated from human adipose tissue expressed surface markers characteristic of ADSCs. ADSCs proliferation and differentiation in chondrogenic direction were observed in both groups compared. The differences were in the spatial arrangement of the cells. In the collagen scaffolds the most cells were on the surface, while in the scaffolds from collagen and gelatin the cells were distributed throughout the whole volume. The 2 Conclusion: The present study suggested the bioink composition based on high concentration collagen (40 mg/ml) and gelatin granules (6.25 mg/ml) for bioprinting of porous-structured hydrogel constructs. The study showed hydrogel to be appropriate for printing and exhibited the properties of a porous material. The hydrogel provided the uniform distribution of ADSCs in the scaffold volume, contributing to their differentiation in the chondrogenic direction. Thus, the suggested bioink composition appears to be a promising material to be used in tissue engineering.
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