Evidence map›Paper›PMID 41426952›Full record

ArticlemicroPublication biology2025

Measurement of NAD(H) concentration using BCA dye and applications to assays of dehydrogenases.

Umanga Rupakheti, Derian Andrew, Sara E Scanlan, Jackson Tester, Christopher E Berndsen

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in microPublication biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Umanga RupakhetiDepartment of Biology, James Madison University, Harrisonburg, Virginia, United States.
Derian AndrewDepartment of Chemistry and Biochemistry, James Madison University, Harrisonburg, Virginia, United States.
Sara E ScanlanDepartment of Chemistry and Biochemistry, James Madison University, Harrisonburg, Virginia, United States.
Jackson TesterBiotechnology Program, James Madison University, Harrisonburg, Virginia, United States.
Christopher E BerndsenDepartment of Chemistry and Biochemistry, James Madison University, Harrisonburg, Virginia, United States.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Dehydrogenases are a widespread enzyme family that play essential roles in all metabolic processes. We observe that one of the substrates of many dehydrogenases, the coenzyme NAD(H), reacts with copper in the bicinchoninic acid assay to produce a color change that can be measured spectroscopically. NADH reacts across a lower concentration range in this assay (<1 μM to ~50 μM) compared to NAD

Identifiers

PMID41426952
PMCPMC12714346

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.