ArticlePhotoniX2025
Real-time wide-field fluorescence lifetime imaging via single-snapshot acquisition for biomedical applications.
Article in PhotoniX, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
6 citing papers in PubMed.
- Fluorescence-lifetime optical electrophysiology in contracting cardiomyocytes.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Enhancing Fluorescence Lifetime Imaging With Differential Transformer.Journal of biophotonics · 2026Article
- Isolating subsurface fluorescence in macroscopic lifetime imaging via high-spatial-frequency structured illumination.JPhys photonics · 2025Article
- Article
- AlliGator: Open Source Fluorescence Lifetime Imaging Analysis in G.bioRxiv : the preprint server for biology · 2025Article
- Depth-Resolved Macroscopic Fluorescence Lifetime Imaging via High-Spatial-Frequency Structured Illumination.bioRxiv : the preprint server for biology · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
17 authors.
Funding
Abstract
Fluorescence lifetime imaging (FLI) is a powerful tool for investigating molecular processes, microenvironmental parameters, and molecular interactions across tissue to (sub-)cellular levels. Despite its established value in biomedical applications, conventional FLI techniques suffer from long acquisition times, limiting their utility in real-time scenarios like fast biological processes and rapid clinical image-guided interventions. Here, we introduce a novel FLI approach that achieves real-time capability through single-snapshot acquisitions by combining a large-format time-gated SPAD array with dual-gate acquisition capability and a rapid lifetime determination algorithm, thus eliminating time-consuming temporal data collection. We demonstrate this method's scalability and versatility across challenging biomedical applications, such as fast neural dynamics (microscale), multimodal 3D volumetric FLI of tumor organoids (mesoscale), and FLI-guided surgical procedures using tissue-mimicking phantoms (macroscale). Overall, this new methodology significantly enhances FLI's temporal and spatial capabilities, enabling rapid dynamic biomedical signal acquisition and seamless integration into clinical workflows, particularly fluorescence-guided surgery. Supplementary Information: The online version contains supplementary material available at 10.1186/s43074-025-00216-0.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.