ArticleMicrobial biotechnology2025
Developing High-Efficiency Electroporation Protocols for Hard-To-Transform Halomonas spp.
Article in Microbial biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Exploitation of Vibrio alginolyticus Mo245 as a dual producer of a glycosaminoglycan and poly(3-hydroxybutyrate) from glycerol.Bioprocess and biosystems engineering · 2026Article
- Establishing a reference cultivation protocol for systematic characterisation of theFrontiers in bioengineering and biotechnology · 2026Article
- Developing High-Efficiency Electroporation Protocols for Hard-To-Transform Halomonas spp.Microbial biotechnology · 2025Article
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Authors and funding
3 authors.
Funding
Abstract
Halomonas species have recently emerged as attractive candidates for next-generation industrial biotechnology, due to their ability to thrive under high-salt conditions, which allows for fermentation under open, unsterile conditions. However, their genetic manipulation has long been hindered by difficulties in genetic transformation. In this study, we report the development of a highly efficient electroporation protocol for Halomonas elongata DSM 2581. By optimising competent cell preparation and electroporation parameters, and using plasmid DNA purified from dam/dcm methylation-deficient Escherichia coli, we achieved a maximum transformation efficiency of 2.8 × 10
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