ArticleJournal of assisted reproduction and genetics2026
Single-Molecule approach to quantify sperm single-stranded DNA damage using the novel sperm repair-assisted damage detection (SRADD) assay.
Article in Journal of assisted reproduction and genetics, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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6 authors.
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Abstract
purposeSperm DNA damage is linked to male infertility and poor reproductive outcome. Single-stranded DNA (ssDNA) damage in sperm is the most common type of damage yet is not specifically targeted by the commonly used DNA damage assays. This study aimed to quantitatively detect sperm ssDNA damage using a novel, direct method.
methodsThe sperm repair-assisted damage detection (SRADD) assay is a single-molecule technique that uses specific repair enzymes to excise damaged segments and incorporates fluorescently labeled nucleotides, enabling visualization and quantification of the damage. We assessed ssDNA damage in sperm donors following induced damage using varying concentrations of H
resultsSRADD demonstrated high inter-slide reproducibility (ICC = 0.95). Sensitivity was confirmed by quantifying induced damage in a dose-dependent manner (0.5, 1, 1.5 mM of H
conclusionThe SRADD assay quantifies sperm ssDNA with high sensitivity and can process dozens of samples simultaneously, making it valuable for andrology and toxicology research and potentially useful in clinical settings such as sperm banks and male-infertility assessment.
trial registration(6573-19-SMV).
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