ArticleNature communications2025
Emergence of mammalian-adaptive PB2 mutations enhances polymerase activity and pathogenicity of cattle-derived H5N1 influenza A virus.
Article in Nature communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers, 1 of them a synthesis that pooled it.
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Who cites it
11 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Systematic Review and Meta-Analysis of Emerging Viral Zoonotic Diseases, 2016-2026.Transboundary and emerging diseases · 2026Pooled it
- Preclinical characterization of HEC116094, an oral inhibitor of the influenza A virus polymerase PB2 subunit.Emerging microbes & infections · 2026Article
- The mouse-lethal H5N1 influenza virus carrying the PB2-384L/443R/460M characteristics acquired in avian hosts can effectively utilize human ANP32A/B proteins.Emerging microbes & infections · 2026Article
- Mammary Gland Tropism and Milk-Mediated Transmission of H5N1 in Dairy Cattle: Implications for One Health Surveillance.Veterinary sciences · 2026Review
- Isolation and characterization of a clade 2.3.4.4b genotype D1.1 H5N1 virus from dairy cattle in Wisconsin.Journal of virology · 2026Article
- Algorithm-Optimized H5 Influenza mRNA Vaccine Induces Broad Immune Responses.International journal of molecular sciences · 2026Article
- Replication Efficiency of Contemporary Highly Pathogenic Avian Influenza A(H5N1) Virus Isolates in Human Nasal Epithelium Model.Emerging infectious diseases · 2026Article
- Rapid phylogenomic analysis for viral surveillance and metagenomic profiling with Omni2Tree.bioRxiv : the preprint server for biology · 2026Article
- A One Health Computational Framework for Identifying PA Endonuclease Inhibitors Against Contemporary H5N1 Avian Influenza.Veterinary sciences · 2026Article
- Historical Pandemic and Contemporary Influenza A Viruses Reveal PB2 M631L as a Convergent Adaptation to Human ANP32.Microorganisms · 2026Article
- The emergence and molecular evolution of H5N1 influenza viruses in United States dairy cattle.bioRxiv : the preprint server for biology · 2026Article
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10 authors.
Funding
Abstract
Highly pathogenic avian influenza H5N1 poses an increasing public health risk, particularly following its spillover into dairy cows and associated human infections in the U.S. since March 2024. Here, we systematically identified critical PB2 mutations emerged during avian-to-cattle transmission and subsequent adaptation in cattle, notably PB2 M631L, which conferred pathogenicity in mice comparable to the well-characterized PB2 E627K mutation. Retrospective analysis reveals that PB2 631L also circulated in avian and human H5N1 strains during the 2013-2014 outbreaks in Cambodia and Vietnam. Additional adaptive mutations include established markers (E627K, Q591R, D701N), and novel variants (I647V, G685R, K736R). These mutations enhance polymerase activity by improving the utilization of both bovine and human ANP32A proteins, thereby increasing viral fitness and pathogenicity in mammals. The convergence of these adaptations highlights the elevated zoonotic risk of cattle-adapted H5N1 viruses and underscores the urgent need for heightened surveillance across avian and mammalian hosts.
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