Evidence map›Paper›PMID 41421489›Full record

ArticleThe Journal of biological chemistry2026

Protein kinase a regulates cyclooxygenase-2 expression through the RNA-binding proteins HuR and TTP.

Sendi Rafael Adame-Garcia, Thomas S Hoang, Pham Thuy Tien Vo, Valeria Burghi, Rodolfo Daniel Cervantes-Villagrana, Lennis Beatriz Orduña-Castillo, Dana J Ramms, JoAnn Trejo, J Silvio Gutkind

Erratum issuedAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of biological chemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. The COX2-PGEbioRxiv : the preprint server for biology · 2026
    Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors.

Sendi Rafael Adame-GarciaMoores Cancer Center, University of California San Diego, La Jolla, California, USA; Department of Pharmacology, School of Medicine, University of California San Diego, La Jolla, California, USA. Electronic address: sendi.adame.garcia@gmail.com.
Thomas S HoangMoores Cancer Center, University of California San Diego, La Jolla, California, USA; Department of Pharmacology, School of Medicine, University of California San Diego, La Jolla, California, USA.
Pham Thuy Tien VoMoores Cancer Center, University of California San Diego, La Jolla, California, USA; Department of Pharmacology, School of Medicine, University of California San Diego, La Jolla, California, USA.
Valeria BurghiMoores Cancer Center, University of California San Diego, La Jolla, California, USA; Department of Pharmacology, School of Medicine, University of California San Diego, La Jolla, California, USA.
Rodolfo Daniel Cervantes-VillagranaMoores Cancer Center, University of California San Diego, La Jolla, California, USA; Department of Pharmacology, School of Medicine, University of California San Diego, La Jolla, California, USA.
Lennis Beatriz Orduña-CastilloDepartment of Pharmacology, School of Medicine, University of California San Diego, La Jolla, California, USA.
Dana J RammsMoores Cancer Center, University of California San Diego, La Jolla, California, USA; Department of Pharmacology, School of Medicine, University of California San Diego, La Jolla, California, USA.
JoAnn TrejoDepartment of Pharmacology, School of Medicine, University of California San Diego, La Jolla, California, USA.
J Silvio GutkindMoores Cancer Center, University of California San Diego, La Jolla, California, USA; Department of Pharmacology, School of Medicine, University of California San Diego, La Jolla, California, USA. Electronic address: sgutkind@ucsd.edu.

Funding

The Cancer Cell Map Initiative v2.0U54CA274502 · NCI · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI Trey Ideker, Nevan J Krogan · 2022 to 2026
$14.2M
UCSD Cancer Center Training Program in Drug DevelopmentT32CA121938 · NCI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Michael Bouvet, Dwayne G. Stupack · 2006 to 2026
$10.3M
Cell Signaling by Protease-activated G Protein-coupled ReceptorsR35GM127121 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Joann Trejo · 2018 to 2026
$4.5M
NCI NIH HHS T32 CA121938NCI NIH HHS U54 CA274502NIGMS NIH HHS R35 GM127121
6 · The paper itself

Abstract

Cyclooxygenase-2 (COX-2/PTGS2) is an inducible enzyme central to inflammatory responses, and its expression is tightly regulated. Elevated intracellular cAMP levels are known to stimulate COX-2 expression. However, the precise mechanism by which protein kinase A (PKA), the primary cAMP effector, mediates this process remains elusive. In this study, we investigated the role of PKA in regulating COX-2 expression in macrophages. We found that PKA activity is essential for COX-2 expression, primarily through a posttranscriptional mechanism that enhances COX-2 mRNA stability. This effect is mediated by the interaction between PKA and the RNA-binding proteins HuR (ELAVL1) and TTP (tristetraprolin/ZFP36). Specifically, we observed that the catalytic subunit of PKA directly interacts with HuR, Hu antigen R (HuR), a well-established COX-2 mRNA stabilizer. PKA activation increased HuR binding to COX-2 mRNA, and pharmacological inhibition of HuR abrogated COX-2 expression in macrophages stimulated with PGE

Indexed as

Cyclic AMP-Dependent Protein KinasesCyclooxygenase 2ELAV-Like Protein 1Gene Expression Regulation, EnzymologicTristetraprolinAnimalsHumansMacrophagesMiceRAW 264.7 CellsRNA-Binding ProteinsRNA, MessengerRNA StabilityCyclic AMP-Dependent Protein KinasesCyclooxygenase 2ELAVL1 protein, humanElavl1 protein, mouseELAV-Like Protein 1Ptgs2 protein, mouseRNA-Binding ProteinsRNA, MessengerTristetraprolinZfp36 protein, mouseciclooxigenase-2postranscriptional regulationprotein kinase ARNA binding proteinssignal transduction

Identifiers

PMID41421489
PMCPMC12914655

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.