Evidence map›Paper›PMID 41420777›Full record

ArticleBioresources and bioprocessing2025

Integrating real-time OUR monitoring with adaptive feeding for enhanced antibody production.

Xin-Ran Zhang, Qingyuan Ran, Botao Zhang, Yong-Mei He, Liang Zhao, Wen-Song Tan, Qian Ye

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bioresources and bioprocessing, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Xin-Ran ZhangState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, P. O. Box 309#, 200237, China.
Qingyuan RanState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, P. O. Box 309#, 200237, China.
Botao ZhangState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, P. O. Box 309#, 200237, China.
Yong-Mei HeState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, P. O. Box 309#, 200237, China.
Liang ZhaoState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, P. O. Box 309#, 200237, China.
Wen-Song TanState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, P. O. Box 309#, 200237, China.
Qian YeState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, P. O. Box 309#, 200237, China. qy@mail.ecust.edu.cn.ORCID http://orcid.org/0000-0001-9310-1553

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundFed-batch culture is a well-established and widely adopted platform for industrial antibody production using Chinese Hamster Ovary (CHO) cells. However, conventional fixed feeding strategies often fall short in meeting the dynamically changing nutrient demands of cells, leading to metabolic imbalance and suboptimal productivity. Oxygen Uptake Rate (OUR), as a real-time indicator of cellular respiratory activity, is tightly coupled to nutrient metabolism and holds strong potential for guiding adaptive, demand-driven feeding strategies.

methodsTo understand the decline in specific productivity (Q

resultsThe RF strategy resulted in a mismatch between nutrient supply and cellular demand during LSP, triggering osmotic stress and limiting antibody expression. In contrast, the OBCF strategy dynamically aligned nutrient delivery with cellular respiration, thereby mitigating osmotic stress and reshaping intracellular metabolism. Notably, OBCF enhanced pyruvate utilization and TCA cycle activity, promoted amino acid catabolism, and suppressed by-product accumulation. These metabolic improvements led to a 52% increase in specific productivity (Q

Indexed as

Chinese hamster ovary (CHO) cellsContinuous feeding strategyFed-batch cultureOxygen uptake rate (OUR)Process analytical technologies (PAT)

Identifiers

PMID41420777
PMCPMC12718288

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.