Evidence map›Paper›PMID 41420757›Full record

ArticleApplied microbiology and biotechnology2025

Double-antigen sandwich ELISA based on S1 for cross-species detection of Porcine deltacoronavirus antibodies.

Yingjie Bai, Ruiming Yu, Liping Zhang, Zhongwang Zhang, Ya Liu, Dongsheng Wang, Peng Zhou, Zhangfu Pei, Huichen Guo, Li Pan and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Applied microbiology and biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Yingjie BaiState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China.
Ruiming YuState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China.
Liping ZhangState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China.
Zhongwang ZhangState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China.
Ya LiuState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China.
Dongsheng WangState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China.
Peng ZhouState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China.
Zhangfu PeiChia Tai Conti (Meizhou) Nucleus Pig Breeding Co., Ltd, Meizhou, 514000, China.
Huichen GuoState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China.
Li PanState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China.
Xinsheng LiuState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China. liuxinsheng@caas.cn.

Funding

China Agriculture Research System CARS-35Major Science and Technology Project of Gansu Province 24ZD13NA008National Center of Technology Innovation for Pigs NCTIP-XD/C 03
6 · The paper itself

Abstract

Porcine deltacoronavirus (PDCoV) is an emerging enteric coronavirus causing high mortality in neonatal piglets and posing a significant threat to the swine industry. Evidence indicates that PDCoV has cross-species transmission potential and may pose a zoonotic risk, emphasizing the need for reliable serological tools for epidemiological surveillance and vaccine evaluation. Here, we developed a double-antigen sandwich enzyme-linked immunosorbent assay (DAgS-ELISA) based on the PDCoV S1 protein, expressed in CHO cells and used as both coating and HRP-conjugated antigen. The assay reliably detected PDCoV-specific antibodies in sera from pigs, chickens, rabbits, and mice, showing high sensitivity (92.86%) and specificity (99.11%) as determined by receiver operating characteristic curve analysis, with excellent reproducibility. No cross-reactivity was observed with antibodies against other common swine pathogens. Concordance with indirect immunofluorescence assay was 96.18% (kappa = 0.923), and assay results correlated strongly with neutralizing antibody titers (Pearson r = 0.865). Overall, this S1-based DAgS-ELISA provides a sensitive, specific, and cross-species applicable method for PDCoV serological detection, supporting its use for epidemiological surveillance and evaluation of vaccine-induced neutralizing antibodies. KEY POINTS: • A novel S1-based double-antigen sandwich ELISA was established for PDCoV detection. • The assay shows high sensitivity, strong specificity, and broad cross-species applicability. • ELISA results strongly correlate with neutralizing antibody titers, aiding vaccine evaluation.

Indexed as

Antibodies, ViralCoronavirus InfectionsDeltacoronavirusSpike Glycoprotein, CoronavirusSwine DiseasesAnimalsAntibodies, NeutralizingAntigens, ViralChickensCHO CellsCricetulusCross ReactionsEnzyme-Linked Immunosorbent AssayMiceRabbitsReproducibility of ResultsAntibodies, NeutralizingAntibodies, ViralAntigens, ViralSpike Glycoprotein, CoronavirusPorcine deltacoronavirus (PDCoV); S1 truncated protein; double antigens sandwich enzyme-linked immunosorbent assay (DAgS-ELISA)

Identifiers

PMID41420757
PMCPMC12718270

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.