Evidence map›Paper›PMID 41420313›Full record

ArticleEnvironmental microbiology reports2025

A Monitoring Method to Evaluate the Accumulation of Antimicrobial-Resistance Genes in Gram-Negative Bacteria Distributed in Environmental Water.

Nobuyoshi Yagi, Sora Miyazato, Nguyen Quoc Anh, Bui Thi Mai Huong, Itaru Hirai

Abstract read
In one paragraph

Article in Environmental microbiology reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Nobuyoshi YagiLaboratory of Clinical Physiology, School of Health Sciences, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan.ORCID https://orcid.org/0000-0003-2814-4455
Sora MiyazatoLaboratory of Microbiology, School of Health Sciences, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan.ORCID https://orcid.org/0009-0001-1388-260X
Nguyen Quoc AnhDepartment of Food Microbiology and Molecular Biology, National Institute of Nutrition, Hanoi, Vietnam.ORCID https://orcid.org/0000-0001-7298-8060
Bui Thi Mai HuongDepartment of Food Microbiology and Molecular Biology, National Institute of Nutrition, Hanoi, Vietnam.ORCID https://orcid.org/0009-0007-2002-8712
Itaru HiraiLaboratory of Microbiology, School of Health Sciences, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan.ORCID https://orcid.org/0000-0002-7143-9315

Funding

Japan Society for the Promotion of Science KAKENHI 23K09672Japan Society for the Promotion of Science KAKENHI 24K20197
6 · The paper itself

Abstract

Antimicrobial-resistant bacteria (AMRB) distributed in the environment can cause human refractory infections. Sufficient monitoring of environmental AMRB had not been performed regularly, and a less burdensome and more efficient method for monitoring environmental AMRB was needed. Assuming to monitor AMRB in the environment, we selected 910 AMRB isolates using cefotaxime and ciprofloxacin from Vietnamese environmental water samples and subjected to 16S rRNA sequencing. It indicated that Escherichia coli (36.0%), Citrobacter freundii (21.4%), Acinetobacter baumannii (20.6%), and Klebsiella pneumoniae (19.7%) were dominant. Using E. coli as a model, we further analysed AMRB isolates by phylogenetic analysis and whole-genome sequencing (WGS). The sequenced full-length fimH of the isolates were plotted with the already published fimH sequences on a phylogenetic tree. Considering phylogeny, 14 E. coli strains were subjected to WGS that indicated not only the number and type of ARGs but also the order of ARGs on the plasmid were confirmed in the analysed E. coli isolates. More importantly, 3 of the 14 strains were bla

Indexed as

Anti-Bacterial AgentsDrug Resistance, BacterialEnvironmental MonitoringGram-Negative BacteriaWater MicrobiologyCefotaximeEscherichia coliMicrobial Sensitivity TestsPhylogenyPlasmidsRNA, Ribosomal, 16SVietnamWhole Genome SequencingAnti-Bacterial AgentsCefotaximeRNA, Ribosomal, 16Santimicrobial‐resistanceantimicrobial‐resistant bacteriaenvironmentmonitoring

Identifiers

PMID41420313
PMCPMC12717432

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.