ArticleBMC bioinformatics2025
EasyKASP: a simple and fast tool for KASP primer design.
Article in BMC bioinformatics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
1 citing paper in PubMed.
- Development of long InDel markers through whole genome resequencing for variety purity detection in cucumber.Scientific reports · 2026Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
backgroundKompetitive Allele-Specific PCR (KASP) is a fluorescence-based, high-throughput and cost-effective genotyping technology widely used for detecting single nucleotide polymorphisms (SNPs) and insertion-deletions (InDels) across various species. However, few software tools are available for automatically designing KASP primers, especially for InDel variations.
resultsTo address the lack of free and user-friendly automated tools for KASP primer design, we analyzed the sequence characteristics of KASP primers and developed a user-friendly program named EasyKASP on the Excel VBA platform. EasyKASP designs KASP primers for both SNP and InDel variations, with an average processing time of only 0.03 s per primer pair. A total of 80 SNP loci and 6 InDel loci with variations of different lengths were selected to validate the KASP markers designed by EasyKASP, all of which were successfully amplified and genotyped using KASP technology.
conclusionsEasyKASP is a simple and rapid tool for KASP primer design, demonstrating broad applicability in KASP genotyping studies.
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Registered trials
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