ArticleApplied microbiology and biotechnology2025
Identification of strong constitutive promoters in Burkholderia stagnalis TBRC 18363 for activating natural product production in Gram-negative bacteria.
Article in Applied microbiology and biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Gram-negative bacteria are emerging as an important source of natural products with pharmaceutical potential. However, the limited availability of genetic tools for drug discovery and sustainable production of secondary metabolites remains a challenge. Burkholderia spp. serve as a promising source for such tools, as these bacteria produce diverse natural products and are amenable to genetic modification. We sequenced the genome of Burkholderia stagnalis TBRC 18363 and performed transcriptomic analysis to identify genes highly expressed in early to late exponential cultures. We hypothesized that the sequences upstream of the most highly expressed genes contain strong and constitutive promoters active in heterologous Gram-negative hosts. Twenty-six B. stagnalis TBRC 18363 promoters were evaluated in Escherichia coli and Pseudomonas putida reporter systems. Two promoters, p2035 and p5642, exhibited superior performance in both systems. Promoter exchange experiments at biosynthetic gene clusters showed that these promoters can enhance the production titers of icosalide in B. stagnalis TBRC 18363 and FR900359, a G
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